U7752

Urease from Canavalia ensiformis (Jack bean)

• CAS Number: 9002-13-5
• Enzyme Commission number: 3.5.1.5 (BRENDA, IUBMB)
• EC Number: 232-656-0
• MDL number: MFCD00070858
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Properties

• biological source: Canavalia ensiformis
• Quality Level: 200
• form: solid
• mol wt: hexamer 545 kDa; trimer ~272 kDa
• contains: ~20% dithiothreitol
• suitability: suitable for marker for electrophoresis (non-denaturing PAGE)
• storage temp.: −20°C

Description

General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620

Urease is a multi-subunit nickel-dependent metalloenzyme. It is found abundantly in fungi, yeast, plants, and bacteria. It belongs to the superfamily of amidohydrolases and phosphotriesterases. Urease is also found in leguminous seeds.

Application

Urease from Canavalia ensiformis (Jack bean) has been used:

• as a component of the molecular mass standard in electrophoresis
• as a component of the standard in size-exclusion chromatography
• as a reference in native-polyacrylamide gel electrophoresis (PAGE)

Biochem/physiol Actions

Urease is an essential enzyme of the nitrogen cycle. It plays a role in degrading urea into ammonia and carbamate. Urease provides a source of nitrogen for growth to various organisms. It also plays a major role in seed germination and seed chemical defense.

Other Notes

Contains approx. 1 mg protein

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H334,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1