T1270

Thiamine hydrochloride

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture

• Synonym(s): Aneurine hydrochloride, Vitamin B₁ hydrochloride
• Empirical Formula (Hill Notation): C₁₂H₁₇ClN₄OS · HCl
• CAS Number: 67-03-8
• Molecular Weight: 337.27
• Beilstein: 3851771
• EC Number: 200-641-8
• MDL number: MFCD00012780
• UNSPSC Code: 12352207
• eCl@ss: 34058006
• PubChem Substance ID: 24899952
• NACRES: NA.75

Properties

• biological source: synthetic (organic)
• product line: BioReagent
• Assay: ≥99.0% (HPLC)
• form: powder
• mol wt: M_w 337.27 g/mol
• technique(s): cell culture | insect: suitable; cell culture | mammalian: suitable; cell culture | plant: suitable
• color: white
• mp: 250 °C (dec.) (lit.)
• solubility: H₂O: 50 mg/mL, clear, colorless
• application(s): agriculture
• SMILES string: CC1=NC(N)=C(C[N+]2=CSC(CCO)=C2C)C=N1.Cl.[Cl-]
• InChI: 1S/C12H17N4OS.2ClH/c1-8-11(3-4-17)18-7-16(8)6-10-5-14-9(2)15-12(10)13;;/h5,7,17H,3-4,6H2,1-2H3,(H2,13,14,15);2*1H/q+1;;/p-1
• InChI key: DPJRMOMPQZCRJU-UHFFFAOYSA-M

Description

General description

Thiamine or Vitamin B1 is a positively charged and essential constituent for all tissues. It is present at higher levels in skeletal muscles, liver, kidney, heart, and brain. Thiamine is composed of a pyrimidine ring linked to a thiazole ring via a methylene bridge.

Application

Thiamine hydrochloride has been used as a component:

• of the vitamin-concentrated stock solution for the preparation of Hv-Ca medium for culturing Haloferax volcanii
• of the tris-acetate-phosphate (TAP) medium for culturing Chlamydomonas nivalis
• of modified Bold 3N medium for culturing Chlorella minutissima

Biochem/physiol Actions

Thiamine is converted to thiamine diphosphate post-movement into the cellular membranes by thiamine diphosphokinase. Thiamine diphosphate, an active cofactor is essential for the mechanism of many enzymes involved in the citric acid cycle, the glycolytic pathway, the degradation of branched-chain amino acids, and the pentose phosphate pathway. Thiamine is involved in converting food into energy required for functioning the central and peripheral nervous systems. Lower levels of this vitamin are associated with delirium, Wernicke′s encephalopathy, and Wernicke-Korsakoff syndrome. Thiamine hydrochloride is required to support energy metabolism and amino acid synthesis in cultured cells. It is present in many classical and serum-free formulations.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves