SAB4200497
Anti-THY1 antibody, Mouse monoclonal
clone TH350, purified from hybridoma cell culture
Synonym(s):
Anti-CD90, Anti-CDw90, Anti-FLJ33325, Anti-Thy-1 T-cell antigen, Anti-Thy-1 antigen, Anti-Thy-1 cell surface antigen, Anti-thy-1 membrane glycoprotein, Monoclonal Anti-THY1 antibody produced in mouse
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All Photos(3)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
TH350, monoclonal
form
buffered aqueous solution
mol wt
antigen ~27 kDa
species reactivity
mouse, human, rat
concentration
~1.0 mg/mL
technique(s)
ELISA: suitable
flow cytometry: 20-40 μg/test using A549 cells.
immunohistochemistry: suitable
western blot: 2.0-4.0 μg/mL using NT2 total cell extracts.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... THY1(7070)
General description
Monoclonal Anti-THY1 (mouse IgG1 isotype) is derived from the hybridoma TH350 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the N-terminus of human THY1, conjugated to keyhole limpet hemocyanin (KLH). Monoclonal Anti-THY1 recognizes human, rat and mouse Thy-1.
Thy-1 cell surface antigen (THY1) is a glycoprotein that is expressed on the membranes of adult cells, mesenchymal stromal cells and cancer cells. It has a molecular weight of about 25-37kDa. The gene encoding THY1 is localized on human chromosome 11q23.3.
Thy-1 cell surface antigen (THY1) is a glycoprotein that is expressed on the membranes of adult cells, mesenchymal stromal cells and cancer cells. It has a molecular weight of about 25-37kDa. The gene encoding THY1 is localized on human chromosome 11q23.3.
THY-1 is expressed in various cell types, including human fibroblasts, neurons, blood stem cells and endothelial cells as well as murine T cells.
Specificity
Monoclonal Anti-THY1 recognizes human, rat and mouse Thy-1.
Immunogen
synthetic peptide corresponding to a sequence at the N-terminus of human THY1, conjugated to KLH.
Application
Monoclonal Anti-THY1 antibody produced in mouse may be used in:
- immunoblotting
- flow cytometry
- immunofluorescence
Biochem/physiol Actions
THY-1 is also involved in the inhibition of neurite outgrowth, apoptotic signaling, leukocyte and melanoma cell adhesion and migration and fibroblast proliferation and migration. This protein plays a role as a mediator of cell-cell and cell-matrix interactions. Thy-1 is a cell surface marker in identifying local and circulating liver cancer stem cells. This protein′s expression can be regulated by the epigenetic mechanism of promoter region methylation, in which down-regulation of Thy-1 is associated with a more invasive/metastatic clinical phenotype.
Thy-1 cell surface antigen (THY1) has a role in the activation of T-cells, release of neurotransmitters at synapses, enhancement of adhesion of astrocytes, wound healing, fibrogenesis and tumor suppression. It also plays an important role in cell migration and cytoskeletal reorganization.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For extended storage, freeze at -20 0C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Thomas H Barker et al.
Experimental cell research, 295(2), 488-496 (2004-04-20)
The precise biological role of Thy-1, a glycophosphatidyl-inositol (GPI)-linked cell surface glycoprotein in non-caveolar lipid raft microdomains, remains enigmatic. Evidence suggests that Thy-1 affects intracellular signaling through src-family protein kinases, and modulates adhesive and migratory events, such as thymocyte adhesion
Tanya A Rege et al.
Biochimica et biophysica acta, 1763(10), 991-999 (2006-09-26)
Thy-1 is a 25-37 kDa glycosylphosphatidylinositol (GPI)-anchored protein involved in T cell activation, neurite outgrowth, apoptosis, tumor suppression, wound healing, and fibrosis. To mediate these diverse effects, Thy-1 participates in multiple signaling cascades. In this review, we discuss Thy-1 signaling
Zhen Fan Yang et al.
Cancer cell, 13(2), 153-166 (2008-02-05)
This study characterized cancer stem cells (CSCs) in hepatocellular carcinoma (HCC) cell lines, tumor specimens, and blood samples. The CD90+ cells, but not the CD90(-) cells, from HCC cell lines displayed tumorigenic capacity. All the tumor specimens and 91.6% of
A reduction in CD90 (THY-1) expression results in increased differentiation of mesenchymal stromal cells.
Moraes DA, et al.
Stem Cell Research & Therapy, 7 (2016)
Hong Lok Lung et al.
Oncogene, 24(43), 6525-6532 (2005-07-12)
Using oligonucleotide microarray analysis, THY1, mapping close to a previously defined 11q22-23 nasopharyngeal carcinoma (NPC) critical region was identified as showing consistent downregulated expression in the tumour segregants, as compared to their parental tumour-suppressing microcell hybrids (MCHs). Gene expression and
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