MAK046
Lipase Activity Assay Kit
sufficient for 100 colorimetric tests (with glycerol standard)
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric tests (with glycerol standard)
detection method
colorimetric
relevant disease(s)
pulmonary disorders; gastrointestinal diseases; cancer
storage temp.
−20°C
General description
The lipase family of enzymes catalyze the cleavage of the ester bonds of lipids. In mammals, this family includes many critical members including pancreatic, hepatic, endothelial, and lipoprotein lipase. Lipases, such as pancreatic lipase, are critical for the metabolism of lipids. Lipases also play key roles in processes such as cell signaling and inflammation. Measurements of lipase activity are commonly used to screen for pancreatic injury or disease, and to monitor diseases such as cystic fibrosis, celiac disease, and Crohn′s disease.
Application
Lipase Activity Assay Kit has been used to determine the lipase activity in samples.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the detection of lipase activity in biological samples including tissue, cells and serum
Principle
The Lipase Activity Assay kit provides a simple and direct procedure for measuring lipase activity in a variety of samples. Lipase activity is determined using a coupled enzyme reaction, which results in a colorimetric (570 nm) product proportional to the enzymatic activity present. One unit of Lipase is the amount of enzyme that will generate 1.0 μmole of glycerol from triglycerides per minute at 37 °C.
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
Certificates of Analysis (COA)
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Enhanced absorption of long-chain polyunsaturated fatty acids following consumption of functional milk formula, pre-digested with immobilized lipase ex vivo, in an exocrine pancreatic insufficient (EPI) pig model.
Goncharova K, et al.
Journal of functional foods, 34, 422-430 (2017)
Amélie de Vallée et al.
Frontiers in microbiology, 10, 2829-2829 (2019-12-24)
The gray mold fungus Botrytis cinerea is a necrotrophic pathogen able to infect hundreds of host plants, including high-value crops such as grapevine, strawberry and tomato. In order to decipher its infectious strategy, a library of 2,144 mutants was generated
Effect of phenolic hydroxyl groups on inhibitory activities of phenylpropanoid glycosides against lipase.
Wu X, et al.
Journal of functional foods, 38, 510-518 (2017)
Kristina Martinez-Guryn et al.
Cell host & microbe, 23(4), 458-469 (2018-04-13)
The gut microbiota play important roles in lipid metabolism and absorption. However, the contribution of the small bowel microbiota of mammals to these diet-microbe interactions remains unclear. We determine that germ-free (GF) mice are resistant to diet-induced obesity and malabsorb
Stephanie Perkail et al.
Nature communications, 11(1), 3018-3018 (2020-06-17)
Chronic pancreatitis represents a risk factor for the development of pancreatic cancer. We find that heterozygous loss of histone H2A lysine 119 deubiquitinase BAP1 (BRCA1 Associated Protein-1) associates with a history of chronic pancreatitis and occurs in 25% of pancreatic
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