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M6295

Sigma-Aldrich

Monoclonal Anti-Maltose Binding Protein antibody produced in mouse

clone MBP-17, ascites fluid

Synonym(s):

Monoclonal Anti-Maltose Binding Protein, Maltose Binding Protein Antibody, Maltose Binding Protein Antibody - Monoclonal Anti-Maltose Binding Protein antibody produced in mouse, Anti-MBP

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

MBP-17, monoclonal

contains

15 mM sodium azide

technique(s)

dot blot: suitable
indirect ELISA: suitable
western blot: 1:4,000 using purified, recombinant MBP

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Maltose Binding Protein (MBP) is a periplasmic protein specific to bacteria. It has maltodextrin binding site and binds to linear and cyclic maltodextrins. The mature MBP is synthesized from the pre-protein comprising of 26 amino acids, as signal peptide.
The antibody recognizes native as well as denatured-reduced forms of purified MBP and MBP fusion proteins.

Immunogen

Purified, recombinant MBP fusion protein

Application

Endogenous intracellular levels of MBP in salmonella cells were determined by western blot analysis using a monoclonal anti-MBP antibody.
Monoclonal Anti-Maltose Binding Protein antibody produced in mouse has been used in the detection of MBP fusion protein using:
  • western blotting and in vitro pull-down assay in Arabidopsis thaliana and Oryza sativa proteins
  • dot blot assay of MBP recombinant G protein of Oryza sativa
  • C3b complement component binding assay

Biochem/physiol Actions

Maltose Binding Protein (MBP) mediates uptake of maltose. MBP is a substrate for chaperone secB. Fusion of proteins with MBP tag at the N -terminus improves their solubility.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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