G7877

Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

Type VII, ammonium sulfate suspension, ≥200 units/mg protein

• Synonym(s): glucose-6-phosphate dehydrogenase (NADP+), G-6-P-DH, Zwischenferment
• CAS Number: 9001-40-5
• Enzyme Commission number: 1.1.1.49 (BRENDA, IUBMB)
• EC Number: 232-602-6
• MDL number: MFCD00081656
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bakers yeast
• type: Type VII
• form: ammonium sulfate suspension
• specific activity: ≥200 units/mg protein
• mol wt: 128 kDa
• technique(s): cell based assay: suitable
• solubility: water: soluble
• application(s): diagnostic assay manufacturing
• storage temp.: 2-8°C
• Gene Information: Saccharomyces cerevisiae ... ZWF1(855480)

Description

General description

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).

Application

Glucose-6-phosphate dehydrogenase has been used in a study to investigate the effect of dehydroepiandrosterone on cell growth and mitochondrial function in TM-3 cells. Glucose-6-phosphate dehydrogenase has also been used in a study to investigate the incidence of apoptosis and transcript abundance in bovine follicular cells. Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Unit Definition

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.

Physical form

Crystalline suspension in 3.2 M (NH₄)₂SO₄ solution, pH 7.0

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves