G0282
Granulocyte-Macrophage Colony-Stimulating Factor from mouse
GM-CSF, from mouse, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
GM-CSF
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About This Item
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biological source
mouse
Quality Level
recombinant
expressed in E. coli
Assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
≤0.2 ng/mL EC50 (corresponds to ≥5 × 106 units/mg)
quality
endotoxin tested
mol wt
~15 kDa (125 amino acids including N-terminal methionine)
packaging
pkg of 5 and 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/mg
color
white
solubility
water: soluble, clear, colorless
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... CSF2(12981) , Csf2(12981)
Biochem/physiol Actions
Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.
Physical form
Lyophilized from 10 mM acetic acid plus 250 μg BSA.
Analysis Note
The EC50 activity of mouse GM-CSF is tested in culture using murine FDP-1 cells.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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