C3131
Cytochrome c from bovine heart
≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture
Synonym(s):
CYC, CytC
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About This Item
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biological source
bovine heart
Assay
≥95% based on Mol. Wt. 12,327 basis
form
powder
mol wt
12327 Da
storage condition
(Tightly closed Dry)
technique(s)
cell culture | mammalian: suitable
impurities
0.40-0.50% Iron (anhydrous)
solubility
water: 10 mg/mL, dark red-brown
UniProt accession no.
storage temp.
−20°C
Gene Information
cow ... CYC1(512500)
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General description
Research area: Apoptosis
Cytochrome c (Cyt C) is an essential mitochondrial protein located in the inner membrane of the mitochondria. It is encoded in the nucleus as apo-cytochrome C. Cyt C occurs as membrane-bound or as soluble metalloproteins in energy-transducing membranes and is found in bacteria, archaea, and plastids. It belongs to the group of class IIa cytochromes and contains pentacoordinate heme centres.
Cytochrome c (Cyt C) is an essential mitochondrial protein located in the inner membrane of the mitochondria. It is encoded in the nucleus as apo-cytochrome C. Cyt C occurs as membrane-bound or as soluble metalloproteins in energy-transducing membranes and is found in bacteria, archaea, and plastids. It belongs to the group of class IIa cytochromes and contains pentacoordinate heme centres.
Application
Cytochrome C from bovine heart has been used:
- as a component of the assay solution for testing the activity of respiratory chain complexes III and IV in the mitochondria of human osteosarcoma and human hepatocarcinoma cell lines
- to determine the cytochrome c oxidase activity in mitochondria of human aortic endothelial cells
- as a component of the cyt C oxidase reaction solution during in situ enzyme staining of cyt C oxidase in mice kidney tissues
Biochem/physiol Actions
Cytochrome C (Cyt C) is involved in the activation of caspase during the caspase-dependent apoptosis intrinsic pathway that triggers programmed cell death through apoptosis. It shows lipid-binding activity through its interaction with cardiolipin that accounts for the peroxidase activity of Cyt C. Cyt C binds to heme via the help of cytochrome c heme lyase that enables its release into the mitochondrial intermembrane space.
The ready interconversion of cytochrome c between ferrous and ferric states makes it an efficient biological electron carrier. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal link in the respiratory chain, it forms the essential electron-bridge between the respirable substrates and oxygen.
Preparation Note
Prepared with acetic acid without using TCA.
Other Notes
View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.
Application
Product No.
Description
Pricing
antibody
related product
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Protocols
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Chromatograms
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