4B200

Sepharose^™ 4B

45-165 μm bead diameter

• CAS Number: 9012-36-6
• MDL number: MFCD00081294
• UNSPSC Code: 12352200
• PubChem Substance ID: 24873317
• NACRES: NA.21

Properties

• biological source: algae (marine)
• Quality Level: 200
• form: suspension
• technique(s): affinity chromatography: suitable
• bead diameter: 45-165 μm
• pore size: 30,000-50,000 Da fractionation range (Dextrans); 60,000-20,000,000 Da fractionation range (Globular proteins)
• storage temp.: 2-8°C
• InChI: 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
• InChI key: MJQHZNBUODTQTK-WKGBVCLCSA-N

Description

General description

Sepharose^™ 4B is an agarose bead and a polymer of Gal β1,4 [3,6]-anhydro-L-galactose. Sepharose aids in fractionating larger molecules and is devoid of charged saccharides. Presence of 3,6-anhydro-L-galactose resists biological degradation. Hydrogen bonds stabilize the sepharose gel structure. Sepharose is widely used in affinity chromatography by cross linking various ligands on the matrix.

Application

Sepharose^™ 4B has been used:

• to conjugate scorpion venom to be used in affinity chromatography
• for clearing of protein extracts prior to immunoprecipitation
• for gel purification and desalting of phospholipid vesicles
• for adsorption of immunoglobulin G (IgG)

Legal Information

Sepharose is a trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point(F): 100.4 - 109.4 °F - closed cup
• Flash Point(C): 38 - 43 °C - closed cup