03353583910
Roche
DIG Oligonucleotide Tailing Kit, 2nd generation
sufficient for 25 reactions (100 pmol oligonucleotide per assay; 1 ug of a 30-mer oligonucleotide)
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About This Item
UNSPSC Code:
41105500
NACRES:
NA.55
Recommended Products
usage
sufficient for 25 reactions (100 pmol oligonucleotide per assay; 1 ug of a 30-mer oligonucleotide)
Quality Level
manufacturer/tradename
Roche
storage condition
avoid repeated freeze/thaw cycles
greener alternative product characteristics
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General description
DIG Oligonucleotide Tailing Kit, 2nd generation employs the enzyme terminal transferase. It catalyzes the addition of digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) and deoxyadenosine triphosphate (dATP) to the 3′-OH end of oligonucleotides.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Application
DIG Oligonucleotide Tailing Kit, 2nd generation has been used to label oligonucleotide probes in:
- northern blot assay
- in situ hybridization (ISH)
- fluorescence in situ hybridization (FISH)
Features and Benefits
Tailing of oligonucleotides at the 3′-end with DIG-11-dUTP and recombinant Terminal Transferase. Oligonucleotides are tailed with DIG-dUTP and dATP at an average tail length of 50 nucleotides (tail length range: 10 – 100).
- Very sensitive hybridization probes, due to the incorporation of several DIG-nucleotides
- Fast hybridization kinetics, due to the small size of oligonucleotides
- Single-stranded probes, no renaturation during hybridization
- Sequence can be designed according to the experiment
- Specially suited for in situ hybridization; due to their small size, oligonucleotides readily diffuse into fixed tissues and cells
Packaging
1 kit containing 11 components
Principle
DIG-dUTP and dATP are combined at a concentration that gives the highest DIG incorporation into the tail, and optimal spacing of DIG and dATP, to achieve the highest sensitivity in hybridization experiments. DIG-dUTP and dATP are provided as separate solutions to allow greater flexibility in terms of tail length, hapten spacing, and the use of unlabeled nucleotide(s).
Preparation Note
Working concentration: Oligonucleotides
In one standard labeling reaction up to 100 pmol oligonucleotide (1 μg of a 30-mer oligonucleotide) can be applied.
In one standard labeling reaction up to 100 pmol oligonucleotide (1 μg of a 30-mer oligonucleotide) can be applied.
Storage and Stability
Store at -15–-25 °C. (unopened kit)
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- Reaction Buffer 5x concentrated
- CoCl<SUB>2</SUB> Solution 25 mM
- DIG-dUTP Solution 1 mM
- dATP Solution 10 mM
- Recombinant Terminal Transferase 400 U/μl
- Control Oligonucleotide, unlabeled 20 pmol/μl
- Oligonucleotide, DIG-dUTP/dATP tailed 2.5 pmol/μl
- Control DNA 0.25 mg/ml
- Glycogen Solution 20 mg/ml
- DNA Dilution Buffer, 50 μg/ml fish sperm DNA
- Poly(A) Solution 10 mg/ml
See All (11)
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B Inhalation - Repr. 1B
Storage Class Code
6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 3
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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Articles
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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