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ES-101

Sigma-Aldrich

COMPLETE ES CELL MEDIUM W/ 15% FBS AND LIF

Ready to use medium formulated with 15% fetal bovine serum (FBS) and mouse leukemia inhibitory factor (LIF). Each lot is qualified for mouse embryonic stem (ES) cell culture.

Synonym(s):

ES Cell Culture Medium

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75

Quality Level

400

form

liquid

manufacturer/tradename

Specialty Media

technique(s)

cell culture | mammalian: suitable

input

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

General description

Complete ES Cell medium W/ 15% FBS and LIF is suitable for use with mouse embryonic stem (ES) cells. It also works as a complete medium for the growth of primary mouse embryonic fibroblast (PMEF) feeder cells.

Application

Complete ES Cell medium W/ 15% FBS and LIF has been used to culture mouse cranial neural crest cell line (CNCCs). It has also been used to culture mouse embryonic stem cells.

Physical form

Ready to use medium for culture of mouse pluripotent stem (ES and iPS) cells and feeders

Storage and Stability

Store at -20°C. After thaw, the medium may be stored at 2-8°C for up to 1 month. Protect from light.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Issei Saitoh et al.
Cell medicine, 3(1-3), 97-102 (2012-05-08)
Feeder cells are generally required for establishment and maintenance of embryonic stem (ES)/induced pluripotent stem (iPS) cells. Increased demands for generation of those cells carrying various types of vectors (i.e., KO vectors and transgenes) also require feeder cells that confer
Akira Kinoshita et al.
Development (Cambridge, England), 148(16) (2021-08-03)
Mutations in ITPR1 cause ataxia and aniridia in individuals with Gillespie syndrome (GLSP). However, the pathogenic mechanisms underlying aniridia remain unclear. We identified a de novo GLSP mutation hotspot in the 3'-region of ITPR1 in five individuals with GLSP. Furthermore
Abijeet Singh Mehta et al.
Developmental biology, 471, 97-105 (2020-12-20)
During neurulation, cranial neural crest cells (CNCCs) migrate long distances from the neural tube to their terminal site of differentiation. The pathway traveled by the CNCCs defines the blueprint for craniofacial construction, abnormalities of which contribute to three-quarters of human

Protocols

Mouse Embryonic Stem Cell Culture Procedures & Protocols

Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.

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