1.05230

Oil red O (C.I. 26125)

for microscopy Certistain^®

• Synonym(s): Oil red O (C.I. 26125)
• CAS Number: 1320-06-5
• Molecular Weight: 408.5
• MDL number: MFCD00003898
• UNSPSC Code: 41116130
• EC Index Number: 215-295-3

Properties

• composition: Dye, ≥80% spectrophotometric assay
• mp: 182-186 °C
• bulk density: 270 kg/m³
• εmax: 725-910 at 528-534 nm in chloroform at 0.01 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-
• InChI key: HJAYEODIXUYVIC-LTFAAIOASA-N

Description

General description

The dry dye Oil red O (C.I. 26125) for microscopy Certistain^®, is used for the preparation of a staining solution that can be used for the detection of lipids in histological cryo sections. As a Certistain^® dye it is chemically quality-certified according to highest standards and strict specifications. Oil red O staining is used for the detection of neutral fat or polyethylene granula in e. g. histological tissues, in joint pathology or in cytological sample material. This Oil red O solution is ready-to-use and results in a brilliant, bright red staining of lipids, while the nuclei are counterstained blue with Hematoxylin solution acc. to Gill II (Cat. no. 105175).
The staining of lipids in histology specimens is a physical process. Oil red O is a lipophilic dye; due to its solubility characteristics it diffuses to the tissue lipids. It is registered as IVD and certified as CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Application

Certistain^® dry dye used to detect lipids in cryosections

Analysis Note

Dye content (spectrophotometrically)≥ 80 %Identity (UV/VIS-Spectrum)passes testAbsorption maximum λ max (chloroform)528 - 534 nmSpec. Absorptivity A 1%/1cm (λ_max; 0.01 g/l; chloroform)725 - 910TLC-Testpasses testLoss on drying (110 °C)≤ 8 %Suitability for microscopypasses test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany