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W4128

Sigma-Aldrich

Williams′ Medium E

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Williams’ E medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

Williams′ Medium E, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
phenol red: 0.0107 g/L
glucose: 2.0 g/L (Dextro)
NaHCO3: 2.2 g/L

shipped in

ambient

storage temp.

2-8°C

General description

Williams′ Medium E has been shown to support the growth in long-term culture of adult liver epithelial cells.

Application

Williams′ Medium E has been used to culture primary human hepatocytes and to maintain hepatocellular carcinoma (JHH-6) cell lines.

Reconstitution

Supplement with 0.292 g/L L-glutamine.

also commonly purchased with this product

Product No.
Description
Pricing

supplement

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mandal S, et al.
Soft Matter, 7, 9424-9434 (2011)
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Precision cut liver slices (PCLSs) retain the structure and cellular composition of the native liver and represent an improved system to study liver fibrosis compared to two-dimensional mono- or co-cultures. The aim of this study was to develop a bioreactor
Florian Douam et al.
PLoS pathogens, 14(3), e1006908-e1006908 (2018-03-06)
Amino-acid coevolution can be referred to mutational compensatory patterns preserving the function of a protein. Viral envelope glycoproteins, which mediate entry of enveloped viruses into their host cells, are shaped by coevolution signals that confer to viruses the plasticity to
Charlie Colin-Pierre et al.
Biomolecules, 11(2) (2021-02-13)
The expression of glypicans in different hair follicle (HF) compartments and their potential roles during hair shaft growth are still poorly understood. Heparan sulfate proteoglycan (HSPG) distribution in HFs is classically investigated by conventional histology, biochemical analysis, and immunohistochemistry. In
Isolation and long-term cell culture of epithelial-like cells from rat liver.
G M Williams et al.
Experimental cell research, 69(1), 106-112 (1971-11-01)
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