SRP0126

DNMT1 Active human

recombinant, expressed in baculovirus infected insect cells, ≥50% (SDS-PAGE)

• Synonym(s): CXXC-type zinc finger protein 9, DNA (cytosine-5-)-methyltransferase 1, DNA MTase HsaI, MCMT
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• tag: GST tagged (N-terminal)
• Assay: ≥50% (SDS-PAGE)
• form: aqueous solution
• potency: ≥50
• mol wt: 211 kDa
• packaging: pkg of 10 μg
• manufacturer/tradename: Sigma-Aldrich
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 0.12 mg/mL
• technique(s): inhibition assay: suitable
• NCBI accession no.: NM_001130823
• UniProt accession no.: P26358
• application(s): life science and biopharma
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... DNMT1(1786)

Description

General description

Research area: Cell cycle

DNMT1 (DNA methyltransferase 1) is a member of the DNMT enzyme family which also includes DNMT2, DNMT3A, and DNMT3B. It is a multidomain protein composed of 1616 amino acids. It has a methyltransferase domain in its C-terminal, which shows sequence homology to bacterial methyltransferases. The DNMT1 gene is mapped to human chromosome 19p13.2.

The DNMT1 gene is mapped to human chromosome 19p13.2.

Application

DNMT1 (DNA methyltransferase 1) has been used for RNA electrophoretic mobility shift assay (REMSA) to determine the role of extra-coding RNAs (ecRNAs) in controlling neuronal DNA methylation, through interaction with DNA methyltransferases.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

DNMT1 (DNA methyltransferase 1) is the predominant enzyme that is responsible for maintaining the level of DNA methylation. It is responsible for replicating CpG methylation patterns from parent to daughter DNA strands, thus, creating heritable methylation signatures through cell division. It, therefore, maintains epigenetic state of DNA. Abnormalities in the expression of this protein are linked with the progression and prognosis of multiple cancers, such as hepatocellular carcinoma, pancreatic, lung and bladder cancers. Up-regulation of this protein in GC (gastric cancer) with less differentiation, advanced stage and increased rate of mortality. Upregulation of the DNMT1 gene indicates poor prognosis in malignant cancers including renal cell carcinoma, lymphoma, pancreatic and and bladder cancer.

Unit Definition

One unit is defined as the amount of enzyme required to methylate 1 pmol of DNA substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable