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SML3688

Sigma-Aldrich

Hypoxia probe 4

≥95% (HPLC)

Synonym(s):

5-Methoxy-1,2-dimethyl-3-(((3-oxo-9-(O-tolyl)-3H-xanthen-6-yl)oxy)methyl)-1H-indole-4,7-dione

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About This Item

Empirical Formula (Hill Notation):
C32H25NO6
Molecular Weight:
519.54
UNSPSC Code:
41116158
UNSPSC Code:
12352200
NACRES:
NA.77

Quality Level

Assay

≥95% (HPLC)

form

powder

storage condition

protect from light

color

, Faint yellow to yellow-brown

solubility

DMSO: 2 mg/mL, clear

shipped in

wet ice

storage temp.

-10 to -25°C

SMILES string

CN1C(C(C=C2OC)=O)=C(C2=O)C(COC3=CC=C(C(C4=CC=CC=C4C)=C5C=C6)C(OC5=CC6=O)=C3)=C1C

Biochem/physiol Actions

Hypoxia probe 4 is a Tokyo green-based non-fluorescent hypoxia probe that undergoes cytochrome P450 reductase (PH51)-catalyzed reduction to yield the fluorescent reporter Tokyo green (ex 480 nm, em 515 nm) in conditions of 0.5% O2 and lower, allowing imaging hypoxia levels similar to those detected by EF5 without the use of antibodies. When employed together with resorufin-based hypoxia probe 2 (ex 545 nm, em 582 nm), the probe pair allows the simultaneous (two-color) imaging of different hypoxia levels in living cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Two Color Imaging of Different Hypoxia Levels in Cancer Cells
Journal of the American Chemical Society, 145(4), 2572-2583 (2023)

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