O8264

Anti-ORAI1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-CRACM1, Anti-Calcium release-activated calcium channel protein 1, Anti-TMEM142A, Anti-Transmembrane protein 142A
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~35 kDa (non-glycosylated); antigen ~50 kDa (glycosylated)
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): western blot: 1-2 μg/mL using whole extract of human HEK-293T cells expressing ORAI1
• UniProt accession no.: Q96D31
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ORAI1(84876) ; mouse ... Orai1(109305)

Description

General description

ORAI calcium release-activated calcium modulator 1 (ORAI1) is the pore-forming subunit of the Ca²⁺ release-activated (CRAC) channel. The protein possesses four predicted transmembrane domains and intracellular amino and carboxyl-terminal domains. The ORAI1 gene is localized on human chromosome 12q24.31.

Immunogen

synthetic peptide corresponding to amino acids 288-301 of human ORAI1, conjugated to KLH. The corresponding sequence differs by 2 amino acids in rat and mouse.

Application

Anti-ORAI1 antibody produced in rabbit has been used in immunohistochemistry and Western blotting.

Biochem/physiol Actions

ORAI calcium release-activated calcium modulator 1 (ORAI1) modulates Ca²⁺ entry into cells. The channel is activated by stromal interaction molecule-1 (STIM-1). Mutation in the gene encoding the protein has been linked to late-onset tubular aggregate myopathy and congenital miosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves