MSST0036

SILu^™Lite CCL2 C-C motif chemokine 2 human

recombinant, expressed in HEK 293 cells, MS Protein Standard

• Synonym(s): HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1(MCP-1), Monocyte secretory protein JE, Monocytechemoattractantprotein1, Monocytechemotacticandactivatingfactor(MCAF), Small-inducible cytokine A2
• UNSPSC Code: 23201100
• NACRES: NA.12

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• Assay: ≥98% (SDS-PAGE)
• form: lyophilized powder
• potency: ≥98% Heavy amino acids incorporation efficiency by MS
• technique(s): mass spectrometry (MS): suitable
• suitability: suitable for mass spectrometry (internal calibrator)
• UniProt accession no.: P13500
• storage temp.: −20°C
• Gene Information: human ... CCL2(6347)

Description

General description

CCL2, the most thoroughly characterized CC chemokine, is a monocyte-specific chemoattractant that was demonstrated to attract T lymphocytes and NK cells, but not neutrophils. Enhanced expression of CCL2 was demonstrated in a variety of pathologic conditions, associated with inflammation and mononuclear cell infiltration. Extensive experimental evidence suggests that CCL2 is highly expressed in atherosclerotic plaques. In a recent study, CCL2 was shown to have value as a biomarker for risk stratification in both the initial and the chronic phases after an acute coronary syndromes (ACS). In contrast to CRP, CCL2 levels may be an indicator of long-term cardiovascular risk rather than a marker of acute prognosis. In urine, CCL2 levels were directly associated to Systemic Lupus Erythematosus disease activity, making it a non-invasive biomarker (avoiding blood sampling or tissue biopsies).

Immunogen

QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKTDYKDDDDKGHHHHHHHHGGQ

Biochem/physiol Actions

SILu^™Lite CCL2 is a recombinant human protein expressed in human 293 cells. It is a homodimer consisting of 96 amino acids (including C-terminal polyhistidine and FLAG^® tags), with a calculated molecular mass of 11 kDa. SILu Lite CCL2 is an analytical standard designed to be used as starting material for preparation of calibrators and controls in LC-MS applications.

Physical form

Lyophilized from a solution of phosphate buffered saline.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

SILu is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable