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Sigma-Aldrich

CellVue® Claret Far Red Fluorescent Cell Linker Mini Kit for General Membrane Labeling

Distributed for Phanos Technologies

Synonym(s):

Far red membrane labeling kit

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.32

packaging

pkg of 1 kit

manufacturer/tradename

Distributed for Phanos Technologies

storage condition

protect from light

technique(s)

flow cytometry: suitable

fluorescence

λex 655 nm; λem 675 nm (CellVue claret dye)

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

ambient

storage temp.

room temp

Related Categories

Application

CellVue Claret kit fluoresces in the far red and may be used for general cell membrane labeling including in vitro cell labeling, in vitro lymphocyte proliferation studies,† in vivo cytotoxicity assays,† and also as a second color for monitoring cell-cell membrane transfer.†
CellVue® Claret Far Red Fluorescent Cell Linker Mini Kit for General Membrane Labeling has been used:
  • to label monocyte-derived macrophages (MDMs) for antibody-dependent cellular phagocytosis assay
  • in labeling of Jurkat cells after the induction of apoptosi
  • in labeling of cells in mice for assaying the retention of tumor cells in lung

Biochem/physiol Actions

The CellVue Claret Fluorescent Cell Linker kit uses a proprietary membrane labeling technology to stably incorporate a far red fluorescent dye with long aliphatic tails (CellVue Claret) into lipid regions of the cell membrane.†

Packaging

This kit provides enough dye for up to 25 samples of 20 million cells each and enough diluent for up to 5 samples of 20 million cells each, when using a 2 mL final staining volume containing a final concentration of 2 x 10-6 M of PKH26 dye. Users must determine the optimal dye and cell concentrations from their cell type(s) and experimental purposes.

Linkage

For additional technical details on Fluorescent Cell Linker Dyes including a comprehensive bibliography, please visit PKH and CellVue®; Claret Fluorescent Cell Linker Dyes.

Legal Information

Distributed for Phanos Technologies, Inc.
Patent Information
CellVue is a registered trademark of Phanos Technologies

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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ZAP70 expression directly promotes chronic lymphocytic leukaemia cell adhesion to bone marrow stromal cells.
Sandrine T Lafarge et al.
British journal of haematology, 168(1), 139-142 (2014-08-05)
Roberts Rimsa et al.
Micromachines, 12(5) (2021-06-03)
Current in vitro models have significant limitations for new respiratory disease research and rapid drug repurposing. Lung on a chip (LOAC) technology offers a potential solution to these problems. However, these devices typically are fabricated from polydimethylsiloxane (PDMS), which has
Comparative assessment of clinically utilized CD20-directed antibodies in chronic lymphocytic leukemia cells reveals divergent NK cell, monocyte, and macrophage properties
Rafiq S, et al.
Journal of immunology (Baltimore, Md. : 1950), 190(6), 2702-2711 (2013)
Justin L Spinner et al.
Journal of leukocyte biology, 95(3), 389-398 (2013-11-15)
Yersinia pestis, the bacterial agent of plague, is transmitted by fleas. The bite of an infected flea deposits Y. pestis into the dermis and triggers recruitment of innate immune cells, including phagocytic PMNs. Y. pestis can subvert this PMN response
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