M6435

Methionine Aminopeptidase from Pyrococcus furiosus

≥93% (SDS-PAGE), recombinant, expressed in E. coli

• Enzyme Commission number: 3.4.11.18 (BRENDA, IUBMB)
• MDL number: MFCD03457470
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• Assay: ≥93% (SDS-PAGE)
• form: solution
• specific activity: 0.5 units/mg protein
• mol wt: 37 kDa by SDS-PAGE
• UniProt accession no.: P56218
• foreign activity: Other proteases, none detected
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: Pyrococcus furiosus DSM 3638 ... PF0541(1468383)

Description

General description

Methionine aminopeptidase from Pyrococcus furiosus is a 32 kDa thermostable enzyme.[1] It belongs to type 2a class of methionine aminopeptidase. Methionine aminopeptidase maintains protein homeostasis and coordinates posttranslational modification of proteins in eukaryotes.[2]

X-ray crystallography of the structure of methionine aminopeptidase from Pyrococcus furiosus or PfMAP was performed at a resolution of 1.75A and showed that the protein consists of a catalytic domain containing two cobalt ions in the active site and a unique insertion domain which is specific to the prokaryotic form of the protein. [3]

Application

Methionine Aminopeptidase from Pyrococcus furiosus has been used in a study to analyze the binding of Co(II)-specific inhibitors to the methionyl aminopeptidases from Escherichia coli and Pyrococcus furiosus. [4] It has also been used in a study to examine the binding of a new class of pseudopeptide analog inhibitors. [5]

Biochem/physiol Actions

Thermostable methionine aminopeptidase, which specifically liberates the N-terminal methioinine from proteins and peptides.

Unit Definition

One unit will hydrolyze 1 μmol of Met from Met-Pro-Ala-Ala-Gly in 1 minute at pH 7.5 at 37 °C.

Physical form

Solution containing 0.01% Tween^® 20, 0.1 mM CoCl₂, and 10 mM Tris-HCl, pH 7.5.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable