I2002

Isocitric Dehydrogenase (NADP) from porcine heart

Type IV, buffered aqueous glycerol solution, 3-20 units/mg protein

• Synonym(s): threo-D_s-Isocitrate: NADP⁺ oxidoreductase, ICDH, IDH
• CAS Number: 9028-48-2
• Enzyme Commission number: 1.1.1.42 (BRENDA, IUBMB)
• EC Number: 200-289-5
• MDL number: MFCD00131420
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Type IV
• form: buffered aqueous glycerol solution
• quality: ≤ 0.2% of main activity is obtained with NAD as cofactor.
• specific activity: 3-20 units/mg protein
• composition: Protein biuret
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Application

Isocitric dehydrogenase (IDH) has been used:

• for in vitro activity assay to study the effects of thorium on citric acid cycle enzymes in the mitochondrial respiration pathway
• as a standard to measure the impact of temperature on nicotinamide diphosphate (NADP)-IDH activity
• in isocitrate assay

Biochem/physiol Actions

Isocitrate dehydrogenase (IDH) is a homodimeric enzyme and participates in several processes such as adapting to hypoxia, histone demethylation, and DNA modification. This enzyme has three isoforms like IDH₁, IDH₂, and IDH₃.

Unit Definition

One unit will convert 1.0 μmole of isocitrate to α-ketoglutarate per min at pH 7.4 at 37 °C.

Physical form

Solution in 50% glycerol in EDTA buffer salts, pH 6.0

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves