A5043

Adenosine Deaminase from bovine spleen

Type X, buffered aqueous glycerol solution, ≥130 units/mg protein

• Synonym(s): ADA, adenosine aminohydrolase, Adenosine aminohydrolase
• CAS Number: 9026-93-1
• Enzyme Commission number: 3.5.4.4 (BRENDA, IUBMB)
• MDL number: MFCD00081287
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bovine spleen
• Quality Level: 200
• type: Type X
• form: buffered aqueous glycerol solution
• specific activity: ≥130 units/mg protein
• mol wt: 32.5-33 kDa
• cation traces: NH₄⁺: ≤10 μg/mg protein
• UniProt accession no.: P56658
• foreign activity: alkaline phosphatase ≤0.01%; guanase ≤0.01%; nucleoside phosphorylase ≤0.1%
• storage temp.: 2-8°C
• Gene Information: cow ... ADA(280712)

Description

General description

Molecular weight: 32.5-33 kDa

pI: 4.85

Application

Adenosine Deaminase from bovine spleen has been used in the immobilization on biostrip for stability and catalytic studies.

Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Biochem/physiol Actions

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

Unit Definition

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Physical form

Solution in 50% glycerol, 5 mM potassium phosphate, pH 6.0

Analysis Note

Protein determined by biuret.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable