83702

Rhodamine 123

BioReagent, for fluorescence, ≥85% (HPLC)

• Synonym(s): 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester
• Linear Formula: C₂₁H₁₇ClN₂O₃
• CAS Number: 62669-70-9
• Molecular Weight: 380.82
• Beilstein: 6030951
• EC Number: 263-687-8
• MDL number: MFCD00012664
• UNSPSC Code: 12352116
• PubChem Substance ID: 57652966
• NACRES: NA.32

Properties

• grade: for fluorescence
• product line: BioReagent
• Assay: ≥85% (HPLC)
• form: powder
• impurities: ≤10% water
• solubility: ethanol: 20 mg/mL, clear, red
• suitability: in accordance for fluorescence
• SMILES string: Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24
• InChI: 1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H
• InChI key: MYFATKRONKHHQL-UHFFFAOYSA-N

Description

General description

Rhodamine 123 is a fluorescent dye.

Application

Rhodamine 123 has been used:

• as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays
• to label the cells for estimating the P-gp expression by flow cytometry
• in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)
• the detection of mitochondrial membrane potential (ΔΨm)

Biochem/physiol Actions

Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.

Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.

Other Notes

Supravital mitochondrial-specific dye

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves