70540

Naphthol Yellow S

suitable for microscopy

• Synonym(s): 2,4-Dinitro-1-naphthol-7-sulfonic acid sodium salt, 5,7-Dinitro-8-hydroxy-2-naphthalenesulfonic acid sodium salt, Acid Yellow 1, Flavianic acid sodium salt
• Empirical Formula (Hill Notation): C₁₀H₄N₂Na₂O₈S
• CAS Number: 846-70-8
• Molecular Weight: 358.19
• EC Number: 212-690-2
• UNSPSC Code: 12171500
• NACRES: NA.47
• PubChem Substance ID: 329762555
• MDL number: MFCD00003958
• Colour Index Number: 10316
• Beilstein/REAXYS Number: 3839220

Properties

• form: powder
• Quality Level: 200
• solubility: methanol: water (1:1): 0.5 g/10 mL, clear
• εmax: ≥275 at 423-433 nm in water, ≥≥ 250 at 387-397 nm in water
• suitability: suitable for microscopy
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[O-]c1c(cc([N+]([O-])=O)c2ccc(cc12)S([O-])(=O)=O)[N+]([O-])=O
• InChI: 1S/C10H6N2O8S.2Na/c13-10-7-3-5(21(18,19)20)1-2-6(7)8(11(14)15)4-9(10)12(16)17;;/h1-4,13H,(H,18,19,20);;/q;2*+1/p-2
• InChI key: CTIQLGJVGNGFEW-UHFFFAOYSA-L

Description

Application

Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.

Biochem/physiol Actions

Naphthol yellow S (NYS) is used as a stain for protein basic groups. It is an acidic dye and forms a NYS-protein complex at acidic pH. Combination of feulgen and NYS provides DNA to protein ratio.

Analysis Note

λ_max. ∼428 nm/∼392 nm; E(1%,1 cm) ∼275-425/∼250-400 (H₂O)

Safety Information

• pictograms: GHS08,GHS07
• signalword: Warning
• hcodes: H317,H373
• pcodes: P260 - P272 - P280 - P302 + P352 - P314 - P333 + P313
• Hazard Classifications: Skin Sens. 1 - STOT RE 2
• Storage Class: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)