11427598910

Biotin-16-ddUTP

≥98% (HPLC), solution, 1 mM, suitable for hybridization

• Synonym(s): Bio-16-ddUTP tetralithium salt, 5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′,3′-dideoxyuridine 5′-triphosphate
• Empirical Formula (Hill Notation): C₃₂H₅₂N₇O₁₇P₃S
• CAS Number: 188755-42-2
• Molecular Weight: 931.78
• MDL number: MFCD32671794
• UNSPSC Code: 41116100

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: solution
• mol wt: 955.5 (biotin-16-ddUTP-Li₄)
• packaging: pkg of 25 μL (25 nmol; 1mM)
• manufacturer/tradename: Roche
• concentration: 1 mM
• technique(s): hybridization: suitable
• storage temp.: −20°C

Description

General description

Biotin-16-ddUTP, tetralithium salt; 1mM

Application

• Biotin-16-ddUTP is used as a substrate for: Terminal Transferase
• DNA polymerase I (Holoenzyme and Klenow fragment)
• T4 DNA polymerase
• Taq DNA polymerase and reverse transcriptase (e.g.,Transcriptor).

Biotin-16-ddUTP is preferentially used for 3′-end labeling of oligonucleotides with Terminal Transferase, recombinant and labeling DNA.

• The biotin-labeled oligonucleotide can be used as a hybridization probe for: DNA and RNA transfers
• Colony and plaque screening
• In situ hybridization

In addition to common hybridization techniques, biotin-labeled oligonucleotides are specifically useful for screening expression libraries for sequence-specific DNA-binding proteins, for example, transcription factors.
The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection.
Oligonucleotides are enzymatically labeled at their 3′ end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine- triphosphate (biotin-ddUTP).
It has been used in the biotinylation of in vitro transcribed tmRNA during surface plasmon resonance (SPR) technique. It has been used in the standard nick translation reaction for tumor DNA labelling during comparative genomic hybridization.

Features and Benefits

Biotin is bound to dideoxyuridine triphosphate via an amide linkage.

Quality

Typical analysis: At least 85% Biotin-16-ddUTP (HPLC, area%).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): does not flash
• Flash Point(C): does not flash