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Key Documents

05-406

Sigma-Aldrich

Anti-JAK3 Antibody, clone B32-32

clone B32-32, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

B32-32, monoclonal

species reactivity

human, mouse

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable

isotype

IgG2bκ

NCBI accession no.

NM_000215

UniProt accession no.

P52333

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... JAK3(3718)
mouse ... Jak3(16453)

Specificity

JAK3

Immunogen

GST-fusion protein containing amino acids 1076-1100 of murine thymus JAK3. Clone B32-32.

Application

Anti-JAK3 Antibody, clone B32-32 detects level of JAK3 & has been published & validated for use in IP.
Not recommended for WB.
Research Category
Signaling
Research Sub Category
Immunological Signaling

Quality

routinely evaluated by immunoprecipitation of JAK3 from CTLL cells or from TF-1 RIPA cell lysate

Target description

125kDa

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl containing 0.05% sodium azide
Format: Purified
Protein A chromatography

Storage and Stability

2 years at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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JAK-mediated phosphorylation and activation of STAT signaling proteins. Analysis by phosphotyrosine blotting and EMSA.
N Broughton et al.
Methods in molecular biology (Clifton, N.J.), 124, 131-146 (2000-12-02)
C C Tassou et al.
Journal of applied microbiology, 104(6), 1764-1773 (2008-02-27)
The purpose of this study was to investigate the inactivation kinetics of Staphylococcus aureus in a ham model system by high hydrostatic pressure at ambient (25 degrees C) and selected temperatures (45, 55 degrees C). Selective [Baird Parker (BP) agar]
T L Wong et al.
Journal of applied microbiology, 97(6), 1185-1191 (2004-11-18)
To obtain preliminary data on the microbiology and hurdles to pathogen growth in the traditional Pacific Island food, povi masima, which is essentially beef brisket cured in brine. Six containers of povi masima were prepared and two were inoculated with
Ultraviolet radiation inhibits interleukin-2-induced tyrosine phosphorylation and the activation of STAT5 in T lymphocytes.
Kulms, D; Schwarz, T
The Journal of Biological Chemistry null
C B Gurniak et al.
Oncogene, 13(4), 777-786 (1996-08-15)
Using a PCR-based screen to identify tyrosine kinases involved in T cell development, we have cloned a new member of the Eph-family of receptor tyrosine kinases (Mep, for murine eph-family protein). At the amino acid level Mep is 60% identical
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