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Yes, this product is derived from Agaricus bisporus.
T3824
Tyrosinase from mushroom
Tyrosinase from mushroom
lyophilized powder, ≥1000 unit/mg solid
Synonym(s):
Catechol Oxidase, Monophenol Monooxygenase, Monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, Polyphenol Oxidase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.
Recommended Products
biological source
fungus (mushroom)
Quality Level
form
lyophilized powder
specific activity
≥1000 unit/mg solid
mol wt
119.5 kDa by electrophoresis
128 kDa by sedimentation velocity diffusion
133 kDa by LS
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
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General description
Isoelectric point (pI): 4.7-5
pH optimum is 6-7
Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.
Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. The enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper. The copper is probably Cu(I), with inactivation involving oxidation to Cu(II) ion.
pH optimum is 6-7
Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.
Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. The enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper. The copper is probably Cu(I), with inactivation involving oxidation to Cu(II) ion.
Tyrosinase is a copper-containing oxidase with activity for catechols and cresol. Tyrosinase participates in the conversion of tyrosine to dihydroxyphenylalanine (DOPA), and polymerization of DOPA leads to melanin formation. Tyrosinase has a role in browning reactions. The enzyme is said to have two binding sites for aromatic substrates and a distinct binding site for oxygen-copper.
Application
Biochem/physiol Actions
Converts tyrosine to L-DOPA or tyramine to dopamine
Physical properties
Isoelectric point (pI): 4.7-5
pH optimum is 6-7
Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.
pH optimum is 6-7
Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.
Unit Definition
One unit = ΔA280 of 0.001 per min at pH 6.5 at 25 °C in 3 mL reaction mix containing L-tyrosine.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Certificates of Analysis (COA)
Lot/Batch Number
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Is this tyrosinase is derived from Agaricus bisporus or another mushroom species?
1 answer-
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Can you please tell me 25000 units tyrosinase in milligrams?
1 answer-
This product is sold by activity. The minimum activity specification for this material is 1000 units per milligram. The weight of this product can be determined using the activity value reported on the lot specific Certificate of Analysis(COA). For example, the sample COA has a activity value of 6540 units/mg. The amount of powder in the 25,000 unit package will be 3.82mg.
Please see the link below or navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a sample or lot specific Certificate:
https://www.sigmaaldrich.com/US/en/product/sigma/t3824#product-documentationHelpful?
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Hi, How much is the total protein amount of T3824 (25KU) per vial?
1 answer-
The protein content of this product is not determined. This product is tested and released based on the activity per milligram solid. Should the protein content be required, please see the link below to review several kits that may be of interest:
https://www.sigmaaldrich.com/US/en/product/compare?term=total%20protein%20kit&facet%5B0%5D=facet_product_category%3Akits&focus=products&page=1&perpage=30&sort=relevance&type=product&products=TP0300%3ASIGMA%3Asial%2CTP0200%3ASIGMA%3Asial%2CTP0100%3ASIGMA%3AsialHelpful?
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What solution is best to be used to reconstitute the T3824-25KU? and what is the volume and concetration of that soluation?
1 answer-
Tyrosinase is soluble at 2 mg/mL in 50 mM potassium phosphate buffer. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/377/683/t3824dat-ms.pdfHelpful?
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I used to order another tyrosinase (Product No. T7755), which has since been discontinued. What is the difference between the replacement product T3824 and the discontinued product T7755?
1 answer-
The difference between these two products is that T3824 has been stabilized with trehalose, while T7755 was not.
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I see that the approximate activity of Product T3824, Tyrosinase from mushroom, is ?1000 unit/mg solid, but how can I find the lot specific activity of this tyrosinase?
1 answer-
The certificate of analysis for the lot will report the exact tyrosinase activity for a particular batch. The lot number can be found on the label of the bottle, or you can call technical service (800-325-5832) for a sample lot number to view the online certificate of analysis.
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How can Tyrosinase from mushroom, Product T3824, be dissolved and what is its expected stability in solution?
1 answer-
Tyrosinase can be dissolved in 50 mM potassium phosphate buffer at 2 mg/mL. Solutions are expected to retain activity for several days in the refrigerator (2-8°C) and for several weeks in the freezer (-20°C).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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The website says Product T3824, Tyrosinase from mushroom, should be stored at -20°C, but I received it at room temperature. Is this correct?
1 answer-
The product is shipped at ambient temperature and is stable for up to two weeks during shipment. Once received, it is recommended to store the product at -20°C to insure the quality for long term storage.
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