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T1378

Sigma-Aldrich

Sigma 7-9®

≥99% (titration), crystalline, Sigma 7-9®

Synonym(s):

Trizma® base, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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About This Item

Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
MDL number:
UNSPSC Code:
12352104
PubChem Substance ID:
NACRES:
NA.25

product name

Sigma 7-9®, ≥99% (titration), crystalline

description

aminopeptidase substrate

Assay

≥99% (titration)

form

crystalline

storage condition

dry at room temperature

technique(s)

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable

impurities

≤0.5% water (Karl Fischer)

color

white to off-white

pH

10.5-12

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: 0.667 g/mL, clear, colorless to faintly yellow

absorption

≤0.4 at 290 at 40%

application(s)

clinical research
diagnostic assay manufacturing
life science and biopharma
sample preparation

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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General description

Sigma 7-9® also known as Trizma base or tris(hydroxymethyl)aminomethane, is a versatile biological buffer used in various applications, such as protein freezing, electrophoresis, and the preparation of buffer solutions such as TAE and TBE. With a pKa of 8.06, Tris is particularly valuable in labs working within the typical physiological pH range of living organisms (pH 7.0-9.0). It is also commonly employed in combination with other buffering agents to precisely adjust solution pH.

Additionally, Tris aids in enhancing the permeability of cell membranes. This compound is indispensable in laboratories where it is utilized to prepare buffers compatible with biological fluids, serve as a standard pH solution, and facilitate procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells.

Application

Sigma 7-9® has been used:

  • as a buffer for sample preparation in a study involving Enzyme-linked immunosorbent assay (ELISA)
  • as a buffer for sample preparation in research involving immunohistochemistry and immunofluorescence

Features and Benefits

  • Suitable for Biological and Biochemical Research
  • Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
For additional information on our range of Biochemicals, please complete this form.

Legal Information

Sigma 7-9 is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sofie Forsberg Soerensen et al.
Frontiers in neurology, 12, 703249-703249 (2021-08-10)
Background: Optic neuritis (ON) is a common inflammatory optic neuropathy, which often occurs in neuromyelitis optica spectrum disease (NMOSD). An experimental model of NMOSD-ON may provide insight into disease mechanisms. Objective: To examine the pathogenicity of autoantibodies targeting the astrocyte
Qian Liu et al.
Cell & bioscience, 13(1), 138-138 (2023-07-29)
Gamma sensory stimulation may reduce AD-specific pathology. Yet, the efficacy of alternating electrical current stimulation in animal models of AD is unknown, and prior research has not addressed intensity-dependent effects. The intensity-dependent effect of gamma electrical stimulation (GES) with a
Sylvain Clède et al.
Chemical communications (Cambridge, England), 48(62), 7729-7731 (2012-06-29)
A rhenium tris-carbonyl derivative has been designed to couple infrared and luminescent detection in cells. Both spectroscopies are consistent with one another; they point out the reliability of the present SCoMPI (for Single Core Multimodal Probe for Imaging) for bimodal
Corey A Baron et al.
Magnetic resonance in medicine, 73(3), 1075-1084 (2014-04-12)
An acquisition method that does not increase scan time or specific absorption rate is investigated for reducing the deleterious effects of cerebrospinal fluid (CSF) partial volume effects on diffusion tensor imaging (DTI) tractography. It is based on using a shorter
Filippo Bertoli et al.
Small (Weinheim an der Bergstrasse, Germany), 10(16), 3307-3315 (2014-04-17)
Nanoparticles in contact with cells and living organisms generate quite novel interactions at the interface between the nanoparticle surface and the surrounding biological environment. However, a detailed time resolved molecular level description of the evolving interactions as nanoparticles are internalized

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