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RAB0715

Sigma-Aldrich

Human MB / Myoglobin ELISA Kit

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.82 ng/mL
standard curve range: 0.819-200 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MB(4151)

General description

The MB ELISA kit provides for the Quantitative measurement of Myoglobin in Cell Culture Supernatants, Plasma and Serum.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Myoglobin is a hemoprotein that regulates the storage and diffusion of oxygen in heart and skeletal muscles. It also protects the tissues from oxidative damage by controlling the levels of reactive oxygen species and nitric oxide. Myoglobin modulates nitric oxide and oxygen levels in the mitochondrial compartments of skeletal muscle and cardiac cells.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Fangfei Li et al.
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Xiaoyun Liu et al.
International journal of cardiology, 296, 129-135 (2019-08-24)
The therapeutic potential of doxorubicin (DOX) is limited by cardiotoxicity. Rubicon is an inhibitory interacting partner of autophagy protein UVRAG. Currently, the role of Rubicon in DOX-induced cardiotoxicity is unknown. In this study, we test the hypothesis that loss of
Malte Krueger et al.
Cytokine, 113, 277-284 (2018-07-23)
This study examined the acute effects of a single session of Whole-body Cryotherapy (WBC) following severe intermittent running exercise on biomarkers of inflammation, muscle damage and stress. Endurance-trained males (n = 11) were tested twice using a within-participant, balanced cross-over design that
Unmasking the Janus face of myoglobin in health and disease.
Hendgen-Cotta UB, et al.
The Journal of Experimental Biology, 213(16), 2734-2740 (2010)
Myoglobin: an essential hemoprotein in striated muscle.
Ordway GA, et al.
The Journal of Experimental Biology, 207(20), 3441-3446 (2004)

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