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P6534

Phospholipase A2 from porcine pancreas

ammonium sulfate suspension, ≥600 units/mg protein

Synonym(s):

Lecithinase A, PLA2, Phosphatidylcholine 2-acylhydrolase

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-637-7
MDL number:
EC Number:
Specific activity:
≥600 units/mg protein
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form

ammonium sulfate suspension

Quality Segment

specific activity

≥600 units/mg protein

UniProt accession no.

storage temp.

2-8°C

Gene Information

General description

Phospholipase A2 is a small disulfide-rich protein having 124 residues.[1][2] It is a calcium-dependent enzyme.[3]

Application

Phospholipase A2 has been used in phospholipase assay and to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation.[4][5]

Biochem/physiol Actions

It has a high catalytic activity on aggregated substrates compared to monomeric substrates.[3]
Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution, pH 5.5

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 at 37 °C.

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This Item
P8913L0382L7525
specific activity

≥600 units/mg protein

specific activity

≥20 units/mg protein

specific activity

≥20,000 units/mg protein

specific activity

≥200 units/mg protein

Gene Information

pig ... PLA2G1B(445525)

Gene Information

cow ... PLA2G1B(282457)

Gene Information

-

Gene Information

pig ... LDHA(407245)

form

ammonium sulfate suspension

form

lyophilized powder

form

lyophilized powder

form

ammonium sulfate suspension

UniProt accession no.

P00592

UniProt accession no.

P00593

UniProt accession no.

-

UniProt accession no.

P00339

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200


Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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