The patent with number 5,665,328, filed in 1992, lists Paul Karl Horan (PKH) as the main inventor.
MIDI67
PKH67 Green Fluorescent Cell Linker Midi Kit for General Cell Membrane Labeling
Distributed for Phanos Technologies
Synonym(s):
Green PKH membrane midi labeling kit
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352207
Pricing and availability is not currently available.
packaging
pkg of 1 kit
Quality Segment
manufacturer/tradename
Distributed for Phanos Technologies
storage condition
protect from light
technique(s)
flow cytometry: suitable
fluorescence
λex 490 nm; λem 502 nm (PKH67 dye)
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
ambient
storage temp.
room temp
General description
This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.
Application
Other Notes
For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.
Legal Information
CellVue is a registered trademark of Phanos Technologies
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This Item | |||
|---|---|---|---|
| technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable | technique(s) - | technique(s) flow cytometry: suitable |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit |
| fluorescence λex 490 nm; λem 502 nm (PKH67 dye) | fluorescence λex 490 nm; λem 502 nm (PKH67 dye) | fluorescence λex 490 nm; λem 502 nm (PKH67 dye) | fluorescence λex 551 nm; λem 567 nm (PKH26 dye) |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| application(s) cell analysis | application(s) cell analysis | application(s) - | application(s) cell analysis |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
Kit Components Only
Product No.
Description
- PKH67 Dye 2 x 0.1
- Diluent C 6 x 10
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Storage Class
3 - Flammable liquids
flash_point_f
57.2 °F - closed cup
flash_point_c
14 °C - closed cup
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What is the meaning of the abbreviation PKH?
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What is the length of the aliphatic tail for the PKH dyes?
1 answer-
The PKH dyes have a long aliphatic tail that inserts into the membrane, with PKH26 > PKH67 > PKH2. The total numbers of carbons in the alkyl tails for PKH26 are greater than for PKH67, which are in turn greater than for PKH2. The exact tail structures are regarded as proprietary information.
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