MAK442

Sorbitol Assay Kit

Sufficient for 100 colorimetric tests

• Synonym(s): D-Sorbitol Assay Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 100
• input: biological sample(s); food(s); plant sample(s)
• application(s): agriculture; cosmetics; environmental; food and beverages
• detection method: colorimetric
• relevant disease(s): diabetes
• storage temp.: −20°C

Description

General description

Sorbitol, also known as glucitol, is a sugar alcohol metabolized slowly within the human body. It is derived from glucose through the reduction of an aldehyde group to a hydroxyl group. Excessive accumulation of sorbitol in erythrocytes, retinal cells, and Schwann cells has been linked to various health issues including retinopathy, cataracts, peripheral neuropathy, and diabetes. Sorbitol is primarily sourced from corn syrup and naturally occurs in fruits such as apples, pears, peaches, and prunes. Widely utilized as a sugar substitute and laxative, sorbitol also finds applications in specialty culture media, as well as in healthcare, food, and cosmetic products. Monitoring sorbitol levels in biological samples aids in understanding metabolic pathways and tracking the progression of diabetes.

Application

The Sorbitol Assay Kit may be used for:

• Metabolism Research
• Food and Beverage Analysis
• Diabetes Research

Features and Benefits

• Fast and Precise Measurement: Achieve results in less than 1 hour, providing rapid analysis with a detection range of 5 - 1000 μM D-sorbitol ensures accurate measurements across a wide range of concentrations.
• High-Throughput Compatibility: Effortlessly integrate our kit into high-throughput handling systems, optimizing efficiency and ensuring accurate results.

Suitability

The kit is suitable for the quantitative determination of D-sorbitol and evaluation of drug effects on sorbitol metabolism in biological (e.g. blood), food, beverage and agriculture samples.

Principle

The sorbitol assay involves an end-point enzyme coupled MTT/NAD reaction that forms a colored product with an absorption maximum at A₅₆₅ nm. The increase in absorbance at A₅₆₅ nm is directly proportional to the sorbitol concentration.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids