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Minimum Essential Medium Eagle

Name: Minimum Essential Medium Eagle
Brand: SIGMA

With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

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Properties

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
Earle’s salts (5% CO₂): yes
NaHCO₃: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

Description

General description

It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Minimum Essential Medium Eagle consists of inorganic salts, water-soluble vitamins, sugar, and essential amino acids.[1] It is widely used as a media formulation for many cell culture studies.[2] It is a modified version of Basal medium Eagle (BME) and the concentrations of the majority of amino acids are twice as compared to BME.[1]

Application

Minimum Essential Medium Eagle has been used to culture osteoblast-like cell line MG-63, dispersed ventricular cells[3], and endometrial adenocarcinoma Ishikawa cells.[4]

Preparation Note

Formulated to contain 9.6 grams of powder per liter of medium.

Supplement with 2.2 g/L sodium bicarbonate.

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This Item	M7278	M3024	M4526
Sigma-Aldrich M0643 Minimum Essential Medium Eagle	Sigma-Aldrich M7278 Minimum Essential Medium Eagle	Sigma-Aldrich M3024 Minimum Essential Medium Eagle	Sigma-Aldrich M4526 Minimum Essential Medium Eagle
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form powder	form liquid	form powder	form liquid
components HEPES: no Earle’s salts (5% CO₂): yes NaHCO₃: no phenol red: yes L-glutamine: yes sodium pyruvate: no	components L-glutamine: no phenol red: yes HEPES: 25 mM sodium pyruvate: no Earle’s salts (5% CO₂): yes NaHCO₃: yes	components Earle’s salts (5% CO₂): yes L-glutamine: no HEPES: no NaHCO₃: no sodium pyruvate: no phenol red: no	components Earle’s salts (5% CO₂): yes sodium pyruvate: yes glucose: yes L-glutamine: no HEPES: no Hanks’ salts (2% CO₂): no phenol red: yes NaHCO₃: yes stable glutamine: no
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 500	Quality Level 400	Quality Level 500	Quality Level 400

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Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000500472

25D291

0000446770

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Global Trade Item Number

SKU	GTIN
M0643-10L	04061833993750
M0643-50K	04061834181491
M0643-50L	04061833993767
M0643-1L	04061837459504
M0643-10X1L	04061835568802

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