This product is not tested for solubility. For activity testing purposes, it is solubilized at 500 - 1000 units per mL in cold deionized water.
A similar product, L3294, Lipase from Thermus flavus, may be dissolved in 50 mM Tris-HCl, pH 8.0.
Note that this may yield a slightly hazy solution. The solution is expected to be stable for at least one month at 2–8 °C. This information may also be applied to L8525, Lipase from Candida rugosa.
L8525
Lipase from Candida rugosa
lyophilized powder, ≥40,000 units/mg protein
Synonym(s):
Triacylglycerol acylhydrolase, Triacylglycerol lipase
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About This Item
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biological source
fungus (Candida rugosa)
Quality Level
form
lyophilized powder
specific activity
≥40,000 units/mg protein
composition
Protein, ≥4.0%
foreign activity
α-amylase ≤0.05 unit/mg solid
protease ≤0.001 units/mg solid
storage temp.
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
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General description
Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.[1]
Application
Lipase from C. rugosa is used to catalyze the synthesis of flavor esters such as pentyl propanoate and butyl butanoate used in food enhancement. [2]
Lipase from Candida rugosa has been used in a study to assess the influence of phase segregation on enzyme hydrolytic activity. [3]
Lipase from Candida rugosa has been used:
- in the hydrolysis of loaded polycaprolactone–20% tricalcium phosphate (PCL–TCP) composites[4]
- in adsorption of proteins in emulsan/alginate beads and in enzyme assays[5]
- to catalyse methanolysis and hydrolysis reactions[6]
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Biochem/physiol Actions
Lipases are used in detergent, food, leather, pharmaceutical and diary industries.[1] It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.[7]
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Unit Definition
One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37 °C using olive oil (30 minute incubation).
Analysis Note
Protein determined by biuret.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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A M Brzozowski et al.
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Lipases are hydrolytic enzymes which break down triacylglycerides into free fatty acids and glycerols. They have been classified as serine hydrolases owing to their inhibition by diethyl p-nitrophenyl phosphate. Lipase activity is greatly increased at the lipid-water interface, a phenomenon
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