F8924

Fibroblast Growth Factor-10 human

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): FGF-10
• MDL number: MFCD01867039
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in E. coli
• Assay: >97% (SDS-PAGE)
• form: lyophilized powder
• potency: 28 ng/mL
• mol wt: 19 kDa by SDS-PAGE
• packaging: pkg of 25 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: O15520
• storage temp.: −20°C
• Gene Information: human ... FGF10(2255)

Description

General description

FGF10 (fibroblast growth factor 10) is a member of the FGF protein family, which is a mitogen of epithelial origin and has a paracrine activity. It is produced by mesenchymal lineage cells, and acts as a ligand for FGFR, most notably FGFR2b, which is expressed by epithelial cells.[1]

Application

Fibroblast Growth Factor-10 human is suitable for culturing of HUVEC cell line (human umbilical vein endothelial cells) to determine the anti-angiogenic properties of sFLT-1 (soluble fms-related tyrosine kinase-1).[2]

Biochem/physiol Actions

FGF10 (fibroblast growth factor 10) is involved in cell growth, morphogenesis and embryogenesis. It plays an essential role in initiating the formation of limb bud, lung morphogenesis and brain formation during embryogenesis. It acts as a mitogen, where it facilitates the regeneration of epithelia post-bladder urothelial injury. Thus, it might have future applications during bladder transplantation.[1] Studies in Japanese population show that polymosphisms- rs339501, rs12517396, and rs10462070 are linked with severe myopia, and might act as susceptibility factors for the same.[3] In lung cancer cells, FGFR4 reduces the stem cell/self-renewal property of cancer cells, and makes them more susceptible to chemotherapy.[4]

Physical form

Lyophilized from a 0.2 μm filtered solution in 20 mM MOPS, 50 mM sodium sulfate, 1 mM DTT, 0.5 mM EDTA, pH 7.2, containing 50 μg bovine serum albumin per 1 μg cytokine.

Analysis Note

The biological activity is measured by its ability to stimulate the proliferation of a monkey epithelial cell line 4MBr-5.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable