E1388

β-Endothelial Cell Growth Factor human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): ECGS, β-ECGF
• CAS Number: 105843-51-4
• MDL number: MFCD00131012
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• product name: β-Endothelial Cell Growth Factor human, β-ECGF, recombinant, expressed in E. coli, suitable for cell culture
• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.03-0.3 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• mol wt: predicted mol wt ~17 kDa
• packaging: pkg of 25 μg
• storage condition: avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1.0 EU/μg protein Endotoxin level (LAL test)
• UniProt accession no.: P49765
• storage temp.: −20°C
• Gene Information: human ... VEGFB(7423)

Description

General description

The β-endothelial cell growth factor (b-ECGF) gene encodes an angiogenic factor, and is mapped to human chromosome 11q13.1. Recombinant, human b-endothelial cell growth factor (b-ECGF) is a 14 amino acid N-terminal extension of acidic fibroblast growth factor. It is expressed in Escherichia coli. The 154 amino acid recombinant protein, generated after removal of the initiation methionine, has a predicted molecular mass of about 17 kDa.

Application

β-Endothelial Cell Growth Factor human has been used:

• in cell death assay
• in endothelial cell culture
• in human umbilical vascular endothelial cell (HUVEC) culture

Biochem/physiol Actions

β-Endothelial cell growth factor (β-ECGF) is a potent endothelial cell mitogen and its mitogenic activity is potentiated by heparin. β-ECGF stimulates the growth of quiescent HUVEC cells in culture. β-ECGF is associated with lipid and glucose metabolism. This growth factor induces neovascularization in disease conditions. Upregulation of β-ECGF is observed in diabetic retinopathy, and polycystic ovary syndrome. It is also associated with tumor proliferation and invasion. β-ECGF increases fatty acid transport proteins expression to improve endothelial mediated lipid transpor.

Other Notes

14 amino acid, N-terminal extended form of human FGF-acidic.

Physical form

Lyophilized from a 0.2 μm filtered solution in 10 mM MOPS, 50 mM ammonium sulfate, 0.2 mM DTT, and 0.2 mM EDTA, pH 7.2, containing 50 μg bovine serum albumin per 1 μg cytokine.

Analysis Note

The proliferative activity is tested in culture by using quiescent NR6R-3T3 fibroblasts.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves