If not used immediately, parafilm wrap the ECM coated culture plates and store at 2-8°C until ready to
use. Use the ECM coated culture plates within 3-4 days.
E1270
ECM Gel from Engelbreth-Holm-Swarm murine sarcoma
ECM Gel from Engelbreth-Holm-Swarm murine sarcoma
liquid, BioReagent, suitable for cell culture
Synonym(s):
Basement membrane extract (BME), EHS matrix
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About This Item
Pricing and availability is not currently available.
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product line
BioReagent
Quality Level
form
liquid
concentration
8-12 mg/mL
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−20°C
Gene Information
mouse ... Lama1(16772) , Lama3(16774) , Lama5(16776) , Lamb1-1(16777) , Lamc1(226519) , Lamc2(16782) , Nid1(18073) , Sdc2(15529)
Application
ECM gel can used with epithelial cells, endothelial cells, muscle cells, nerve cells and tumor cells.
Biochem/physiol Actions
The ECM gel will undergo thermally activated polymerization when brought to 20-40°C, forming a reconstituted basement membrane. PC12 cells will show neurite formation within 2 days when they are grown on a thin layer of this ECM gel.
Every mouse colony used for the production of this product is routinely screened for several pathogens. Tested and found negative for LDEV.
Every mouse colony used for the production of this product is routinely screened for several pathogens. Tested and found negative for LDEV.
Components
ECM was prepared to a protein concentration of 8-12 mg/mL (in DMEM), containing laminin as a major component, collagen type IV, heparin sulfate proteoglycan, entactin, and other minor components. Addition of collagen type IV to the ECM gel increases polymerization.
Product contains 50 mg/L Gentamicin Sulfate.
Product contains 50 mg/L Gentamicin Sulfate.
Caution
For long term storage, keep product at -20°C. The ECM gel may be stored at 2-8°C for up to 72 hours.
Preparation Note
This product is prepared from Engelbreth-Holm-Swarm sarcoma in mice; dialyzed against chloroform. Gel should be thawed overnight at 2-8°C before use and dispensed to a multiwall plate using a plate and pipettes that are pre-cooled to 2-8°C. The gel may also be diluted up to twofold with 2-8°C Dulbecco′s Modified Eagle′s Medium, and should be done before gel is added to the plate. The product will gel within 5 minutes at 20°C. For prolonged manipulations, work should be conducted below 10°C. Cells can be plated on top of a thin gel layer of 0.5 mm or cultured inside a 1 mm layer. If you culture cells inside, add the cells to the gel prior to plating at a recommended density of 3-4 x 104 cells per mL. To dissociate cells from this gel, use protease in PBS without calcium, magnesium, or EDTA, at a concentration of 0.6-2.4 units per mL.
also commonly purchased with this product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
Cell culture protocols using ECM Gel Matrix EHS basement membrane extract (BME) for neurite outgrowth, cell invasion and angiogenesis tube formation assays.
Cell culture protocols using ECM Gel Matrix EHS basement membrane extract (BME) for neurite outgrowth, cell invasion and angiogenesis tube formation assays.
Cell culture protocols using ECM Gel Matrix EHS basement membrane extract (BME) for neurite outgrowth, cell invasion and angiogenesis tube formation assays.
Cell culture protocols using ECM Gel Matrix EHS basement membrane extract (BME) for neurite outgrowth, cell invasion and angiogenesis tube formation assays.
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Can ECM Gel from Engelbreth-Holm-Swarm murine sarcoma be coated on a plate and stored overnight at 4C in parafilm to be used the next day?
1 answer-
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Is this product suitable for implantation of tumors in mice or does the phenol red preclude it?
1 answer-
Product E1270 Sigma-Aldrich ECM Gel from Engelbreth-Holm-Swarm murine sarcoma has been validated for cell culture applications only. This product is designed for coating tissue culture glassware. It has not been validated for in vivo use.
Please refer to the following link below for more information:
https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/3d-cell-culture/ecm-gel-product-protocolsHelpful?
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Can this be used for xenograft studies in immuncompromised mice.
1 answer-
This product is not tested on xenograft studies in immunocompromised mice. It is not recommended to be used for that application.
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Do Products E1270 and E6909, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, contain phenol red?
1 answer-
Product E1270 ECM gel does contain phenol-red from the Dulbecco's modified Eagle's medium component of the product. Product No. E6909, ECM gel, growth factor reduced, is phenol red free.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Are Products E1270 and E6909, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, growth factor reduced like Matrigel (from BD)?
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Product No. E1270 is not growth factor reduced. Product No. E6909 is ECM gel, growth factor reduced.
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How can I retrieve my cells from Product E1270, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma?
1 answer-
Dispase (Product No. D4818) is a Bacillus-derived neutral protease that is recommended for recovering cells cultured on ECM (Product No. E1270) or Matrigel. Dispase will yield a single cell suspension far more effectively than trypsin, collagenase, or other proteolytic enzymes. Dispase is gentle and will not damage cells harvested for sub-cultivation or other study.
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The data sheet for Product E1270, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, says that it may be diluted up to twofold. What does that mean?
1 answer-
ECM gel can be diluted twofold with cold Dulbecco's Modified Eagle's Medium (DME). This means 1 part ECM gel and 1 part DME.
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What is the calcium concentration in Products E1270, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma?
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The approximate concentration of CaCl2 in the ECM solution is 0.265 mg/mL.
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Are ECM Gels, Products E1270 and E6909, the same as Matrigel (from BD)?
1 answer-
Product No. E1270, ECM gel is the equivalent of Matrigel from BD only produced by Sigma-Aldrich.
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