PA-1

90013101, human ovary, Epithelial

• UNSPSC Code: 41106514

Properties

• product name: PA-1, 90013101
• biological source: human ovary
• growth mode: Adherent
• karyotype: 2n = 46, pseudodiploid
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian ovary teratocarcinoma

Cell Line Description

Derived from ascitic fluid cells of a 12 year old Caucasian female with teratocarcinoma of the ovary. The cells produce tumours in nude mice.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 9,12
D13S317: 9,10
D16S539: 9,12
D5S818: 11
D7S820: 9
THO1: 7,9
TPOX: 11
vWA: 15,17

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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