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C9830

Sigma-Aldrich

Chitosanase from Streptomyces griseus

lyophilized powder, >50 units/mg protein (Bradford)

Synonym(s):

Chitosan N-acetylglucosaminohydrolase

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About This Item

CAS Number:
51570-20-8
Enzyme Commission number:
3.2.1.132 (BRENDA, IUBMB)
MDL number:
MFCD00211797
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

>50 units/mg protein (Bradford)

purified by

chromatography

solubility

H2O: soluble, clear to slightly hazy, colorless (10U/0.3mL)

foreign activity

chitinase <1.0 units/mg protein

storage temp.

−20°C

General description

Chitosanase is synthesized from Streptomyces griseus as an extracellular secreted enzyme and corresponds to a molecular weight of 34 kDa. It has a temperature and pH optimum of 60°C and 5.7, respectively. Chitosanase belongs to the glycosyl hydrolase family and comprises α-helical tertiary structure similar to lysozyme with substrate-binding cleft placed between two lobes.

Application

Chitosanase from Streptomyces griseus has been used in a study to assess the effect of chitin sources on production of chitinase and chitosanase. Chitosanase from Streptomyces griseus has also been used in a study to investigate the effective production of chitinase and chitosanase by Streptomyces griseus HUT 6037 using colloidal chitin and various degrees of deacetylation of chitosan.
Chitosanase from Streptomyces griseus has been used in the digestion of lacrimal gland (LG) tissues.
The enzyme from Sigma has been used for the hydrolysis of the crude cell wall preparation of oomycete Aphanomyces euteiches, a major parasite of legume plants. It has also been used for the enzymatic hydrolysis of the fully de-N-acetylated chitosan to get chitosan oligomer mixtures during the preparation of biocompatible chitosan-alginate gel.

Biochem/physiol Actions

Chitosanase catalyzes the endohydrolysis of N-acetylated chitosan specifically at the of β(1,4) linkages between N-acetyl-D-glucosamine and D-glucosamine residues. Chitosanase from Streptomyces griseus is capable of hydrolyzing both chitosan and carboxymethyl cellulose. Chitosanase is found in several types of microorganisms and is used for the lysis of fungal cell walls.

Unit Definition

One unit will release 1 μmole of glucosamine from chitosan per min at pH 5.0 at 37 °C as measured in the fluorimetric assay of Osswald, et al. (1992).

Physical form

Lyophilized powder containing potassium phosphate buffer salts

Preparation Note

For long term solution storage, reconstitute vial with 50% glycerol solution. Dilute to working concentrations with a 0.1% BSA solution.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Pascal Viens et al.
Marine drugs, 13(11), 6566-6587 (2015-11-01)
Chitosanases, enzymes that catalyze the endo-hydrolysis of glycolytic links in chitosan, are the subject of numerous studies as biotechnological tools to generate low molecular weight chitosan (LMWC) or chitosan oligosaccharides (CHOS) from native, high molecular weight chitosan. Glycoside hydrolases belonging
Chitosanase from Streptomyces griseus.
A Ohtakara
Methods in enzymology, 161, 505-510 (1988-01-01)
Toshiaki Tanabe et al.
Bioscience, biotechnology, and biochemistry, 67(2), 354-364 (2003-05-06)
Streptomyces griseus HUT 6037 inducibly produced two chitosanases when grown on chitosan. To elucidate the mechanism of degradation of chitinous compound by this strain, chitosanases I and II of S. griseus HUT 6037 were purified and characterized. The purified enzymes
Thang Trung Khong et al.
Biomacromolecules, 14(8), 2765-2771 (2013-06-29)
Biocompatible hydrogels are very interesting for applications in, e.g., tissue engineering and for immobilization of cells, such as calcium-alginate gels where the calcium ions form specific interactions with the guluronic acid units. We here report on a new gelling system
Georg Kopplin et al.
RSC advances, 11(23), 13780-13798 (2022-04-16)
Three alginates with fundamentally different block structures, poly-M, poly-G, and poly-MG, have been investigated upon ionic crosslinking with chitosan oligosaccharides (CHOS), using circular dichroism (CD), rheology, and computer simulations, supporting the previously proposed gelling principle of poly-M forming zipper-like junction
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