C8274
Cytohelicase from Helix pomatia
lyophilized powder
Synonym(s):
Snail gut enzyme
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About This Item
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Application
Cytohelicase from Helix pomatia has been used:
- in the enzyme solution for preparing the cell nuclei for immuno fluorescent in situ hybridization (immunoFISH)
- in the enzyme mix to analyze meiotic cells at diakinesis and later stages in anthers
- in the enzyme mix for the analysis of mitosis in anthers
Cytohelicase from Helix pomatia has been used to study the isolation of yeast protoplasts using various preparations of the hepato-pancreatic juice of Helix pomatia.
The enzyme from Sigma has been used for cytohelicase assay. The enzyme assay was used while studying the effects of MNN10 gene-disruption on protein secretion in Kluyveromyces lactis and Saccharomyces cerevisiae cultures.
Biochem/physiol Actions
Cytohelicase from Helix pomatia is used in the preparation of synaptonemal complex spreading techniques along with bovine serum albumin. It plays an important role in the digestion of cell wall. Cytohelicase interacts with chromatin and improves the adhesion of synaptonemal complexes to the hydrophobic plastic film.
Analysis Note
Contains glucanase/laminarinase activity
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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ImmunoFISH: simultaneous visualisation of proteins and DNA sequences gives insight into meiotic processes in nuclei of grasses.
Sepsi A, et al.
Frontiers in Plant Science, 9(7), 1193-1193 (2018)
Improved method of enzyme digestion for root tip cytology.
Lattier JD, et al.
Hortscience: a Publication of the American Society For Horticultural Science Hortscience, 52(7), 1029-1032 (2017)
The effect of bovine serum albumin and cytohelicase on surface-spread synaptonemal complexes of Rye (Secale cereale).
De Jong JH and van Eden J
Stain Technology, 63(4), 213-220 (1988)
M Diatewa et al.
Biochimie, 63(1), 67-69 (1981-01-01)
Conversion of large amounts of Saccharomyces cerevisiae cells to protoplasts is studied using various preparations extracted from Helix pomatia hepato-pancreatic juice. The most favourable yield in two hours incubations (88 per cent) is obtained with 20 ml cytohelicase, a chitinase
Danguole Bartkeviciūte et al.
FEMS yeast research, 4(8), 833-840 (2004-09-29)
Screening for genes affecting super-secreting phenotype of the over-secreting mutant of Kluyveromyces lactis resulted in isolation of the gene named KlMNN10, sharing high homology with Saccharomyces cerevisiae MNN10. The disruption of the KlMNN10 in Kluyveromyces lactis, as well as of
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