C3144

Coenzyme A sodium salt hydrate

cofactor for acyl transfer

• Synonym(s): CoA Na₂
• Empirical Formula (Hill Notation): C₂₁H₃₆N₇O₁₆P₃S · xNa⁺ · yH₂O
• CAS Number: 55672-92-9
• Molecular Weight: 767.53 (anhydrous free acid basis)
• MDL number: MFCD00079020
• UNSPSC Code: 41106305
• PubChem Substance ID: 24892587
• EC Number: 259-747-8
• NACRES: NA.51

Properties

• Quality Segment: 200
• assay: ≥85% (spectrophotometric assay)
• form: powder
• solubility: H₂O: soluble 50 mg/mL, clear, colorless to faintly yellow
• storage temp.: −20°C
• SMILES string: [Na+].CC(C)(COP(O)(=O)OP(O)(=O)OC[C@H]1O[C@H]([C@H](O)[C@@H]1OP(O)([O-])=O)n2cnc3c(N)ncnc23)C(O)C(=O)NCCC(=O)NCCS
• InChI key: SYTRWOCXZXQBPW-CLVRNSBASA-M

Description

General description

Coenzyme A (CoA) is an essential cofactor in living systems and is synthesized from pantothenic acid (vitamin B5), The CoA levels in mitochondria and peroxisomes correspond to 2-5 mM and 0.7 mM, respectively. Cytosolic CoA is in the range of 0.05 mM to 0.14 mM

Application

Coenzyme A is suitable for use in:

• gylcerolipid biosynthesis in porcine adipose tissue[1]
• an assay to measure the level of Alpha-methylacyl-CoA racemase (AMACR) in human blood samples using a nanoparticle electrochemical biosensor[2]
• chloramphenicol acetyltransferase (CAT) assay[3][4]
• the synthesis of palmitoyl-CoA, which is required for palmitoylation and activation of proteins for regulated membrane fusion[5]

Biochem/physiol Actions

Coenzyme A (CoA, CoASH, HSCoA) is a coenzyme that facilitates enzymatic acyl-group transfer reactions and supports the synthesis and oxidation of fatty acids. CoA is involved in the mechanisms of a wide variety of enzymes. In the presence of CoASH, organic carboxylic acids form acyl-CoA thioesters, which facilitates enzyme recognition. The acyl-CoA formed from xenobiotic carboxylic acids can add to the compound′s toxicity, which can lead to cellular metabolic dysfunction.[6] It is involved in the oxidation of pyruvate in the Kreb′s cycle. CoA is needed for metabolic events. The bacterial CoA pathway is targeted for antimicrobial development. It mediates acyl group transfer and carbonyl activation. The CoA and its thioester levels are crucial for cellular homeostasis. CoA is also involved in regulating platelet aggregation and vasoconstriction. It acts as an essential cofactor in enzymatic acetyl transfer reactions.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)