B8438
Alcian Blue solution
1% in 3% acetic acid, pH 2.5
Synonym(s):
Alcian Blue 8XG solution
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About This Item
UNSPSC Code:
12171500
NACRES:
NA.47
Recommended Products
form
liquid
concentration
1% in 3% acetic acid, pH 2.5
color
dark blue to very dark blue
pH range
2.4-2.6
Amax
0.45-0.60 (1:400 in H2O)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Application
Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.
Biochem/physiol Actions
Alcian Blue is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby aiding in the quantitative determination of glycosaminoglycans. It is also used for the staining of bone and cartilage.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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SPECIFIC STAINING OF SULPHATE GROUPS WITH ALCIAN BLUE AT LOW PH.
R LEV et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 12, 309-309 (1964-04-01)
A procedure for differential staining of cartilage and bone in whole formalin-fixed vertebrates.
Wassersug R J.
Stain Technology, 51(2), 131-134 (1976)
Differential staining of acid glycosaminoglycans (mucopolysaccharides) by alcian blue in salt solutions.
J E Scott et al.
Histochemie. Histochemistry. Histochimie, 5(3), 221-233 (1965-10-01)
G F Pierce et al.
The American journal of pathology, 138(3), 629-646 (1991-03-01)
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