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APPA002

Sigma-Aldrich

Green Universal Detection Reagent

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.25

form

solution

Quality Level

technique(s)

microbe id | specific enzyme detection: suitable

shipped in

dry ice

storage temp.

−20°C

General description

GreenUniversal Detection Reagent is formulated with green fluorescent protein (GFP),which is a part of the split fluorescent protein (FP) system that may be usedto quantify the expression level of a tagged protein of interest (POI).

Application

The S1-10 Universal Detection Reagent is provided as a ready-to-use solution for use with the In Vitro "Fold ′n′ Glow" Solubility Assay Kits:

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Stéphanie Cabantous et al.
Nature biotechnology, 23(1), 102-107 (2004-12-08)
Existing protein tagging and detection methods are powerful but have drawbacks. Split protein tags can perturb protein solubility or may not work in living cells. Green fluorescent protein (GFP) fusions can misfold or exhibit altered processing. Fluorogenic biarsenical FLaSH or
G S Waldo et al.
Nature biotechnology, 17(7), 691-695 (1999-07-15)
Formation of the chromophore of green fluorescent protein (GFP) depends on the correct folding of the protein. We constructed a "folding reporter" vector, in which a test protein is expressed as an N-terminal fusion with GFP. Using a test panel

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