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A5384

Aconitase from porcine heart

Synonym(s):

Aconitate hydratase, Citrate (isocitrate) hydrolyase

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About This Item

CAS Number:
9024-25-3
EC Number:
4.2.1.3 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
MFCD00130335
Biological source:
Porcine heart
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biological source

Porcine heart

Quality Segment

200

form

solid

mol wt

~66 kDa by gel filtration

storage temp.

−20°C

Application

Aconitase may be usefull in diabetes research [1]as well as to study structural conservation between iron-responsive element binding proteins (IRE-BP) and isomerases [2].

Biochem/physiol Actions

Aconitase catalyses the stereo-specific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle. Aconitase is inhibited by cyanide, sulfide and copper and mercury at low concentrations. It is competitively inhibited by trans-aconitate. Aconitase contains an internal Fe-S cluster which is responsible for its activity.

Analysis Note

Crude preparation containing aconitase activity

Other Notes

One unit will convert 1.0 μmole of citrate (via cis-aconitate) to isocitrate per min at pH 7.4 at 25 °C.

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I1877C3260E2884
Aconitase from porcine heart

Sigma-Aldrich

A5384

Aconitase from porcine heart

Isocitric Dehydrogenase (NADP) from porcine heart Type I, 0.5-3.0 unit/mg solid (plus numerous enzyme activities associated with porcine heart)

Sigma-Aldrich

I1877

Isocitric Dehydrogenase (NADP) from porcine heart

Citrate Synthase from porcine heart liquid, ≥100 units/mg protein

Sigma-Aldrich

C3260

Citrate Synthase from porcine heart

Esterase from porcine liver ammonium sulfate suspension, ≥150 units/mg protein (biuret)

Sigma-Aldrich

E2884

Esterase from porcine liver

biological source

Porcine heart

biological source

Porcine heart

biological source

Porcine heart

biological source

Porcine liver

form

solid

form

solid

form

liquid

form

ammonium sulfate suspension

mol wt

~66 kDa by gel filtration

mol wt

-

mol wt

-

mol wt

168 kDa

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Questions

  1. what is the units/mg of this product? Or is it not known?

    1 answer

    1. This product is a crude enzyme preparation, the activity is not a determined test specification. Historical data indicates activity may range from 10 - 40 units per gram. Please see the link below to review the available assay protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/192/355/a5384enz.pdf

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