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23479

Sigma-Aldrich

Nitrite/Nitrate Assay Kit, colorimetric

used for detection of nitric oxide metabolites

Synonym(s):

Nitrate/Nitrite Assay Kit, colorimetric

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

Quality Level

100

usage

sufficient for 100 assays

suitability

passes test for application

storage temp.

2-8°C

Related Categories

Application

Nitric oxide (NO), a gaseous paramagnetic radical, is a very important and versatile messenger in biological systems. NO is synthesized from L-arginine by NO synthase (NOS). It has been identified as an endothelial derived relaxation factor (EDRF) and antiplatelet substance. It serves as a neurotransmitter derived from a neutrophile and a cytotoxic substance from an activated macrophage. Although NO′s molecular action in the biological system is very versatile, the most important role of NO is the activation of guanylate cyclase.

The Griess assay is one of the most popular and simplest methods used to detect the NO concentration. The Griess assay′s mechanism is summarized as the azo coupling between diazonium species, which are produced from sulfanilamide with NO2, and naphthylethylenediamine. The NO2/NO3 Assay Kit contains these dyes, a nitrate reductase, an enzyme co-factor, buffer solution and NO2, NO3 solutions as standards. Therefore, total NO metabolites are easily detectable using this kit. The suitable NO2 detection range is from 10 to 100 μM.

Kit Components Only

Product No.
Description
  • NaNO2 Standard Solution (100 mM) 1 bottle
  • NaNO3 Standard Solution (100 mM) 1 bottle
  • Buffer solution (20 mM, pH 7.6) 1 bottle
  • Nitrate reductase (lyophilized) 1 bottle
  • Enzyme Co-factor (lyophilized) 1 bottle
  • Griess Reagent A 1 bottle
  • Griess Reagent B 1 bottle

related product

Product No.
Description
Pricing

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Warning

Hazard Statements

H290,H315,H317,H319,H335

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL9064
BCCL3034
BCCK3359
BCCK7778
BCCK2381

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Fredrik Sjövall et al.
PloS one, 9(5), e97673-e97673 (2014-05-16)
The levels of nitric oxide (NO) and various cytokines are known to be increased during sepsis. These signaling molecules could potentially act as regulators and underlie the enhancement of mitochondrial function described in the later phase of sepsis. Therefore, we
Mohamed Raâfet Ben Khedher et al.
Lipids in health and disease, 16(1), 241-241 (2017-12-14)
Vasculogenic erectile dysfunction (VED) is considered as a common complication among people with type 2 diabetes (T2D). We tested whether changes in fatty acid (FAs) classes measured in erythrocytes are associated with increased risk of diabetic VED along with related
Junyao Yang et al.
Experimental cell research, 363(2), 262-270 (2018-01-22)
The X-box binding protein 1 (XBP1) is a pivotal transcription factor in the endoplasmic reticulum stress response. Our previous studies have proven that XBP1 is involved in vascular endothelial growth factor (VEGF)-mediated endothelial cell (EC) proliferation and angiogenesis. In this
Rong Wang et al.
Scientific reports, 6, 36790-36790 (2016-11-16)
Atherosclerosis is a serious public health concern. Excessive inflammatory responses of vascular cells are considered a pivotal pathogenesis mechanism underlying atherosclerosis development. It is known that Janus kinase/signal transducer and activator of transcription 3 (JAK/STAT3) signalling plays an important role
Iselin Vildmyren et al.
Nutrients, 10(12) (2018-11-25)
Hypertension is the leading risk factor for cardiovascular disease, and prevention of high blood pressure through diet and lifestyle should be a preferred approach. High intake of fish is associated with lower blood pressure, possibly mediated through the proteins since
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Questions

1–5 of 5 Questions  

  1. Are the culture media, DMEM/F12 and DMEM, suitable for this assay kit? Is it recommended to use media with or without FCS when using the kit?

    1 answer

    1. Avoid cell culture media containing NO3, such as Fe(NO)3 present in DMEM/F12. Centrifuge cell or tissue culture media at 1000 * g for 15 minutes to remove insoluble material. Collect the supernatant for testing. Serum or plasma should be centrifuged in an Amicon® Ultra-4 Centrifugal Filter Unit with Ultracel®-10
      membrane (7000 * g, 4 °C, 20 minutes) to remove hemoglobin and proteins. Please see the link below to review the product Technical Bulletin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/118/770/23479dat.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. hi. Is the nitrite kit could be used to detect nitrite in mice plasma and tissue? Thank you!

    1 answer

    1. This kit can be used for the detection of nitrite in blood plasma. Please refer to this document for sample preparation instructions, https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/118/770/23479dat.pdf.

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  5. Can I use a frozen cell culture supernatant or must it be obtained on the same day for the assay?

    1 answer

    1. When using frozen samples, it is recommended to thaw the samples completely, mix well by vortexing and centrifuge prior to use to remove particulates. Note that cell culture medium that contains NO3- as a component (such as RPMI-1640) are not a suitable to detect [NO3- + NO2-]. However, NO2- in the culture medium can be determined using this kit.

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