R1148

Rogosa SL agar

suitable for microbiology, NutriSelect^® Plus

• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 100; 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 5.4±0.2 (25 °C)
• application(s): bioburden testing; clinical testing; food and beverages; microbiology
• storage temp.: 2-8°C
• suitability: selective by low pH for Lactobacillus spp.

Description

Application

Used as a selective medium for cultivation of Lactobacilli.

Components

Ingredients (g/L)
Tryptose, 10.00
Yeast extract, 5.00
Dextrose, 10.00
Arabinose, 5.00
Saccharose, 5.00
Sodium acetate, 15.00
Ammonium citrate, 2.00
Monopotassium phoshate, 6.00
Magnesium sulphate, 0.57
Manganese sulphate, 0.12
Ferrous sulphate, 0.03
Polysorbate 80, 1.00
Agar, 15.00

Preparation Note

Suspend 75 g of Rogosa SL Agar in 1000 ml of distilled water. Boil to dissolve the medium completely. Add 1.32 ml of glacial acetic acid (Cat. No. 45726). Mix thoroughly, and distribute into culture tubes or flasks. Heat to 90-100°C for 2-3 minutes. Cool to 45°C for direct inoculation.
DO NOT AUTOCLAVE.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves