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H1115000

Hyaluronidase

European Pharmacopoeia (EP) Reference Standard

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About This Item

CAS Number:
9001-54-1
EC Number:
232-614-1
UNSPSC Code:
41116107
NACRES:
NA.24
Pricing and availability is not currently available.

grade

pharmaceutical primary standard

manufacturer/tradename

EDQM

application(s)

pharmaceutical (small molecule)

format

neat

storage temp.

2-8°C

SMILES string

O1COc2c1cc(cc2)C(CC(=O)c3cc4c(cc3)OCO4)C(=O)O

InChI

1S/C18H14O7/c19-13(11-2-4-15-17(6-11)25-9-23-15)7-12(18(20)21)10-1-3-14-16(5-10)24-8-22-14/h1-6,12H,7-9H2,(H,20,21)

InChI key

PPEBBOHQEAQKSW-UHFFFAOYSA-N

General description

This product is provided as delivered and specified by the issuing Pharmacopoeia. All information provided in support of this product, including SDS and any product information leaflets have been developed and issued under the Authority of the issuing Pharmacopoeia.
For further information and support please go to the website of the issuing Pharmacopoeia.

Application

Hyaluronidase EP Reference standard, intended for use in laboratory tests only as specifically prescribed in the European Pharmacopoeia.

Packaging

The product is delivered as supplied by the issuing Pharmacopoeia. For the current unit quantity, please visit the EDQM reference substance catalogue.

Other Notes

Sales restrictions may apply.

related product

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

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Na Liu et al.
The Journal of clinical investigation, 129(2), 631-646 (2018-11-16)
Macrophages perform key functions in tissue homeostasis that are influenced by the local tissue environment. Within the tumor microenvironment, tumor-associated macrophages can be altered to acquire properties that enhance tumor growth. Here, we found that lactate, a metabolite found in
Marie-Ming Aynaud et al.
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EWSR1-FLI1, the chimeric oncogene specific for Ewing sarcoma (EwS), induces a cascade of signaling events leading to cell transformation. However, it remains elusive how genetically homogeneous EwS cells can drive the heterogeneity of transcriptional programs. Here, we combine independent component
Hui-Fang Zhu et al.
Cancer biology & therapy, 20(7), 967-978 (2019-03-22)
Nontumour cells in the tumour microenvironment, especially fibroblasts, contribute to tumour progression and metastasis. The occurrence and evolution of colorectal cancer (CRC) is closely related to cancer-associated fibroblasts (CAFs). The aim of this work was to evaluate the effects of
Wei Xiang et al.
Nature communications, 9(1), 2574-2574 (2018-07-04)
Metabolic reprogramming greatly contributes to the regulation of macrophage activation. However, the mechanism of lipid accumulation and the corresponding function in tumor-associated macrophages (TAMs) remain unclear. With primary investigation in colon cancer and confirmation in other cancer models, here we
Seyed Mojtaba Hosseini et al.
Asian journal of neurosurgery, 13(4), 1101-1107 (2018-11-22)
Spinal cord injury (SCI) as one of the most important diseases of central nervous system (CNS) without any definite treatment is still growing in incidence. In addition to trauma, some surgeries such as cardiac and thoracic aorta surgery may result
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Questions

1–5 of 5 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. 1 mL 中にヒアルロニダーゼ活性が 120 単位になるよう、本品を正確に調製するしたいと考えております。具体的な手順をご教示いただけますと幸いです。

    1 answer

    1. This is a product of the European Pharmacopoiea. The current lot activity is reported in the product leaflet, available at the supplier product page.. The specific dilution procedure is described in the associated EP monograph or general chapter. Please see the link below to review the current leaflet:
      https://crs.edqm.eu/db/4DCGI/leaflet?leaflet=H1115000_3.pdf

      Helpful?

  4. Could you please tell me in which solvent this enzyme should be dissolved? Is it PBS or the culture medium?

    1 answer

    1. This is a product of the European Pharmacopoeia (EP) and is intended for use as an analytical standard. Please see the link below to visit the European Pharmacopeia website for more information and to download the product leaflet:
      https://crs.edqm.eu/db/4DCGI/View=H1115000

      The current shipping lot reports an activity of 577 IU/mg and a package size of 50 mg. For analytical purposes, the EP recommends dissolving the proper amount of hyaluronidase in hyaluronidase diluent. The latter consists of mixing 100 mL of pH 6.4 phosphate buffer solution with 100 mL of water and then dissolving 0.140 g of hydrolyzed gelatin in the solution at 37 °C. It is recommended to prepare fresh solutions.

      A similar research grade, H3506 Hyaluronidase from bovine testes, recommends dissolving the product at 1mg/mL in 0,02 M phosphate buffer, pH 7, with 77 mM sodium chloride and 0.01% BSA. For best results, prepare solutions fresh. Please see the link below to review the datasheet for this alternate product:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/296/016/h3506-pis-ms.pdf

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  5. Hi, what is the activity of the hyaluronidase H1115000?

    1 answer

    1. The activity of hyaluronidase in Product No. H1115000 is lot-specific and is listed in the information leaflet hosted on the European Directorate for the Quality of Medicines & HealthCare (EDQM) website at https://crs.edqm.eu/db/4DCGI/View=H1115000.

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