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RSTREP-RO

Roche

Streptavidin, recombinant

liquid, ≥17 units/mg protein, pH range 5.0-9.0

Synonym(s):

streptavidin

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About This Item

UNSPSC Code:
12352202

biological source

Streptomyces sp. ( Streptomyces avidinii)

Quality Level

100

recombinant

expressed in E. coli

form

liquid

specific activity

≥17 units/mg protein

mol wt

~52 kDa

packaging

pkg of 1 mg (11721666001)
pkg of 5 mg (11721674001)

manufacturer/tradename

Roche

concentration

70-90%

technique(s)

protein array: suitable
protein purification: suitable

impurities

6-7% water

pH range

5.0-9.0

pI 

7.4-7.7 (two main bands)

solubility

water: 10 mg/L (mA405=100)

suitability

suitable for immunological tests

UniProt accession no.

P22629

shipped in

cooler

storage temp.

-10 to -25°C

General description

Streptavidin, a highly versatile protein, is naturally produced by Streptomyces avidinii. It exhibits a strong affinity for biotin, forming a non-covalent yet robust binding interaction. This unique property makes streptavidin an ideal choice for various biological detection systems, including western blotting, enzyme-linked immunosorbent assays (ELISA), and immunohistochemistry.

Application

Use Streptavidin, recombinant for coating of solid phases (e.g., microplates, beads etc.) as well with biotin-labeled haptens and antibodies in universal immunological detection systems (streptavidin/biotin system).

Sequence

Streptavidin is a tetramer with a molecular weight of approximately 53 kD consisting of four subunits of approximately 13 kD, each containing a single biotin-binding site.
Each subunit has six tyrosine residues.The protein is free of carbohydrate.

Unit Definition

One unit binds 1 μg D-biotin at pH 8.9.

Physical form

White lyophilizate; lyophilized in a 20 mM potassium phosphate, pH 6.5 solution
Protein: 0.7 ± 0.1mg protein lyophilizate (A282 = 3.1)
KD: 10-15M

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Production of Recombinant Streptavidin and Optimization of Refolding Conditions for Recovery of Biological Activity
Jouybari RM et al.
Reports of Biochemistry and Molecular Biology, 6(2), 178-185 (2018)
Automated combinatorial process for nanofabrication of structures using bioderivatized nanoparticles.
Dehlinger D, et al.
Journal of the Association for Laboratory Automation, 5, 267-276 (2007)
Michael Weber et al.
Nature photonics, 15(5), 361-366 (2021-05-07)
We introduce MINSTED, a fluorophore localization and super-resolution microscopy concept based on stimulated emission depletion (STED) that provides spatial precision and resolution down to the molecular scale. In MINSTED, the intensity minimum of the STED doughnut, and hence the point
John E G McCarthy et al.
Methods in enzymology, 430, 247-264 (2007-10-05)
A growing number of biophysical techniques use immobilized reactants for the quantitative study of macromolecular reactions. Examples of such approaches include surface plasmon resonance, atomic force microscopy, total reflection fluorescence microscopy, and others. Some of these methods have already been
Michael Weber et al.
Nature biotechnology, 41(4), 569-576 (2022-11-08)
Super-resolution techniques have achieved localization precisions in the nanometer regime. Here we report all-optical, room temperature localization of fluorophores with precision in the Ångström range. We built on the concept of MINSTED nanoscopy where precision is increased by encircling the
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