Skip to Content
Merck
All Photos(2)

Documents

11585614910

Roche

DIG-High Prime DNA Labeling and Detection Starter Kit II

greener alternative

sufficient for 12 labeling reactions (10 ng to 3 μg per assay), sufficient for 24 blots (blots of 100 cm2)

Synonym(s):

DIG system, dna labeling

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41105500

usage

sufficient for 12 labeling reactions (10 ng to 3 μg per assay)
sufficient for 24 blots (blots of 100 cm2)

Quality Level

manufacturer/tradename

Roche

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

storage temp.

−20°C

General description

DIG-High Prime DNA Labeling and Detection Starter Kit II is a convenient kit for random-primed labeling of DNA templates with digoxigenin (DIG)-11- deoxyuridine triphosphate (dUTP), alkali-labile and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers and reaction buffer, all in one convenient reagent.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DIG-High Prime DNA Labeling and Detection Starter Kit II has been used in a variety of hybridization techniques:
  • in Southern blots
  • in northern blots
  • in dot blots
  • in colony and plaque hybridizations
  • for all types of filter hybridization
  • for single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley, and wheat

Packaging

1 kit containing 7 components.

Principle

The DIG High Prime DNA Labeling and Detection Starter Kit II uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent chemiluminescence detection by enzyme immunoassay. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves solely as a template for the synthesis of labeled DNA, and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10 ng) with this method.The complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin present in the reaction, are incorporated into the newly synthesized complementary DNA strand.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • DIG-High Prime 5x concentrated

  • DIG-labeled Control DNA, pBR328 (linearized with Bam HI) 5 μg/ml

  • DNA Dilution Buffer

  • Anti-Digoxigenin-AP Conjugate antibody

  • CSPD ready-to-use

  • Blocking Solution 10x concentrated

  • DIG Easy Hyb Granules

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

does not flash

Flash Point(C)

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Eva-Maria Holstein et al.
Cell reports, 7(4), 1259-1269 (2014-05-20)
A large and diverse set of proteins, including CST complex, nonsense mediated decay (NMD), and DNA damage response (DDR) proteins, play important roles at the telomere in mammals and yeast. Here, we report that NMD, like the DDR, affects single-stranded
M Dutt et al.
Plant cell reports, 26(12), 2101-2110 (2007-08-19)
Shoot apical meristem explants of Vitis vinifera "Thompson Seedless" were used for Agrobacterium-mediated genetic transformation. It was determined that the meristems had to be subjected to a dark growth phase then wounded to obtain transgenic plants. Morphological and histological studies
Ling Zhang et al.
International journal of genomics, 2014, 921950-921950 (2014-09-10)
The Delta-12 oleate desaturase gene (FAD2-1), which converts oleic acid into linoleic acid, is the key enzyme determining the fatty acid composition of seed oil. In this study, we inhibited the expression of endogenous Delta-12 oleate desaturase GmFad2-1b gene by
Erum Dilshad et al.
PloS one, 10(10), e0140266-e0140266 (2015-10-09)
The potent antimalarial drug artemisinin has a high cost, since its only viable source to date is Artemisia annua (0.01-0.8% DW). There is therefore an urgent need to design new strategies to increase its production or to find alternative sources.
Emmanuel Ogwok et al.
Journal of virological methods, 169(2), 296-304 (2010-08-10)
Cassava brown streak disease (CBSD), caused by two distinct species, Cassava brown streak Uganda virus (CBSUV) and Cassava brown streak virus (CBSV), is a major constraint to cassava (Manihot esculenta Crantz) production in Africa. Absence of infectious clones of CBSUV

Articles

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service