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Sigma-Aldrich

Yeast Transformation Kit

reagents for introducing plasmid DNA into yeast

Synonym(s):

lithium acetate yeast transformation

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.85

grade

for molecular biology

Quality Level

200

usage

 kit sufficient for >100 standard transformations

technique(s)

transformation: suitable

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Sigma′s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.

Application

Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.

Features and Benefits

  • Easy and ready-to-use
  • Requires as little as 10 ng of plasmid DNA
  • Flexibility for any strain of yeast
  • Sufficient for over 100 standard transformations

Components

The Yeast Transformation Kit contains:
  • Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
  • Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
  • Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
  • Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
  • Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g

Principle

Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).

Kit Components Also Available Separately

Product No.
Description
SDS
  • D9156Deoxyribonucleic acid, single stranded from salmon testes, For hybridization 2 x 1SDS
  • Y1501Yeast Synthetic Drop-out Medium Supplements, without uracil 1 gSDS

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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James R Petrie et al.
PloS one, 7(4), e35214-e35214 (2012-04-24)
Monoacylglycerol acyltransferases (MGATs) are predominantly associated with lipid absorption and resynthesis in the animal intestine where they catalyse the first step in the monoacylglycerol (MAG) pathway by acylating MAG to form diacylglycerol (DAG). Typical plant triacylglycerol (TAG) biosynthesis routes such
Miren Zumárraga et al.
Proteins, 71(1), 250-260 (2007-10-13)
The generation of diversity for directed protein evolution experiments shows an important bottleneck in the in vitro random mutagenesis protocols. Most of them are biased towards specific changes that eventually confer a predicted and conservative mutational spectrum, limiting the exploration
DMSO-enhanced whole cell yeast transformation.
J Hill et al.
Nucleic acids research, 19(20), 5791-5791 (1991-10-25)
Francesco Palma et al.
FEMS microbiology letters, 272(1), 114-119 (2007-06-22)
The TBF-1 is an 11.9-kDa fruiting body specific protein of the Ascomycetes hypogeous fungus Tuber borchii Vittad. found in aqueous extract and the hyphal cell wall. The tbf-1 gene codes a 12-amino acid N-terminal stretch not present in mature protein.
A two-hybrid screen identifies an unconventional role for the intermediate filament peripherin in regulating the subcellular distribution of the SNAP25-interacting protein, SIP30.
Gentil BJ
Journal of Neurochemistry, 131(5), 588-601 (2014)
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Articles

Yeast Transformation Introduction

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

Yeast Transformation Introduction

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

Yeast Transformation Introduction

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

Yeast Transformation Introduction

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

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Protocols

Yeast Transformation Kit

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

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