Skip to Content
Merck
All Photos(1)

Documents

TOX8

Sigma-Aldrich

In Vitro Toxicology Assay Kit, Resazurin based

Synonym(s):

Fluorescent resazurin toxicology assay

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

EC Number:
263-718-5
UNSPSC Code:
12352207
NACRES:
NA.84

form

liquid

usage

1 kit sufficient for 2,000 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

protein quantification: suitable

application(s)

cell analysis
detection

detection method

colorimetric
fluorometric

storage temp.

2-8°C

General description

In Vitro Toxicology Assay Kit (Resazurin based) is designed for fluorometrically or spectrophotometrically determining cell number as a function of metabolic activity using the dye resazurin. The resazurin method is simple, accurate, and reproducible

Application

In Vitro Toxicology Assay Kit, Resazurin based has been used:
  • in cell viability assay of human primary fibroblasts
  • to determine the cell proliferation rate in murine pre-osteoblasts
  • to determine the metabolic activity of microfluidic and monolayer cultures

Biochem/physiol Actions

Bioreduction of the dye reduces the amount of its oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

U J Strotmann et al.
Ecotoxicology and environmental safety, 25(1), 79-89 (1993-02-01)
The dehydrogenase assay with resazurin as an oxidoreduction dye was used for monitoring the performance of six laboratory-scale sewage treatment plants. The dehydrogenase activity measured proved to be a reliable monitoring parameter, although it did not always positively correlate with
A rapid biochemical test for measuring chemical toxicity.
D Liu
Bulletin of environmental contamination and toxicology, 26(2), 145-149 (1981-02-01)
Comparison of several microbiological toxicity screening tests.
Dutka, B.J., et al.
Water Research, 17, 1363-1367 (1983)
Monika Marędziak et al.
Stem cells international, 2016, 2152435-2152435 (2016-03-05)
Tissue regeneration using human adipose derived mesenchymal stem cells (hASCs) has significant potential as a novel treatment for many degenerative bone and joint diseases. Previous studies have established that age negatively affects the proliferation status and the osteogenic and chondrogenic
José Manuel Quesada-Gómez et al.
The Journal of steroid biochemistry and molecular biology, 148, 214-218 (2014-12-03)
The nitrogen-containing or nitrogenous bisphosphonates (N-BF) are currently the main class of drugs used for the treatment of diseases characterized by an increased bone resorption. Preliminary data suggest that N-BF have also direct or indirect anti-tumoral effects, and recent evidence
View All Related Papers

Articles

Quality Control Considerations in Cell Culture

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

Quality Control Considerations in Cell Culture

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

Quality Control Considerations in Cell Culture

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

Quality Control Considerations in Cell Culture

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service